IL-6 ELISA Kit

Produktnummer ABIN921064

Ratte IL-6 ELISA Kit, Colorimetric Assay für die Quantifizierung von Ratte IL-6 und es wurde in 144+ Publikationen zitiert.

Kurzübersicht für IL-6 ELISA Kit (ABIN921064)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Bindungsspezifität: AA 25-211

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 62.5 pg/mL - 4000 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Produktdetails IL-6 ELISA Kit

Untere Nachweisgrenze: 62.5 pg/mL

Verwendungszweck: Sandwich Rat IL-6/Interleukin-6 ELISA Kit to quantitate Rat Il6 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: F25-T211

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 5 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 5%, 6.2%, 5.9%
Inter-Assay Precision (CV%): 5.5%, 7.1%, 7.5%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für IL-6 ELISA Kit (ABIN921064)

Wang, Mao, Yang, Zou, Liu, Liu, Zhang, Xiao, Wang: "Resveratrol protects against early polymicrobial sepsis-induced acute kidney injury through inhibiting endoplasmic reticulum stress-activated NF-κB pathway." in: Oncotarget, Vol. 8, Issue 22, pp. 36449-36461, (2018) (PubMed).

Piao, Liu, Guo, Meng, Gao: "Picroside II Shows Protective Functions for Severe Acute Pancreatitis in Rats by Preventing NF-κB-Dependent Autophagy." in: Oxidative medicine and cellular longevity, Vol. 2017, pp. 7085709, (2018) (PubMed).

Chen, Wu, Chen, Xie, Fang, Hu, Chen, Fu, He: "Omega-3 polyunsaturated fatty acid supplementation attenuates microglial-induced inflammation by inhibiting the HMGB1/TLR4/NF-κB pathway following experimental traumatic brain injury." in: Journal of neuroinflammation, Vol. 14, Issue 1, pp. 143, (2018) (PubMed).

Xiong, Ru, Chen, Tian, Li: "Mitochondrial dysfunction and inflammatory response in the cytotoxicity of NR8383 macrophages induced by fine particulate matter." in: Environmental toxicology and pharmacology, Vol. 55, pp. 1-7, (2018) (PubMed).

Duan, Lu, Wang, Zhang, Mao, Cao, Lin, Zhang, Shuai, Shen: "The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia." in: International journal of nanomedicine, Vol. 12, pp. 6705-6719, (2018) (PubMed).

Tagliari, Campos, Costa-Casagrande, Salvalaggio: "THE IMPACT OF THE USE OF SYMBIOTICS IN THE PROGRESSION OF NONALCOHOLIC FATTY LIVER DISEASE IN A RAT MODEL." in: Arquivos brasileiros de cirurgia digestiva : ABCD = Brazilian archives of digestive surgery, Vol. 30, Issue 3, pp. 211-215, (2018) (PubMed).

Gao, Meng, Cui: "Budesonide instillation immediately after reperfusion ameliorates ischemia/reperfusion-induced injury in the transplanted lung of rat." in: Experimental lung research, Vol. 43, Issue 9-10, pp. 439-446, (2018) (PubMed).

Liao, Zheng, Xie, Zhao, Zeng, Liu, Liu: "Adipose tissue-derived stem cells ameliorate hyperglycemia, insulin resistance and liver fibrosis in the type 2 diabetic rats." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 286, (2018) (PubMed).

Ling, Feng, Wei, Wang, Wang, Zhang, He, Wang, Zeng, Xiong: "Effects of low-intensity pulsed ultrasound (LIPUS)-pretreated human amnion-derived mesenchymal stem cell (hAD-MSC) transplantation on primary ovarian insufficiency in rats." in: Stem cell research & therapy, Vol. 8, Issue 1, pp. 283, (2018) (PubMed).

Zhang, Xu, Hu, Wu, Qin, Zhang: "Anti-inflammatory effects of Lefty-1 in renal tubulointerstitial inflammation via regulation of the NF-κB pathway." in: International journal of molecular medicine, Vol. 41, Issue 3, pp. 1293-1304, (2018) (PubMed).

Vidal, Cascão, Finnilä, Lopes, Saarakkala, Zioupos, Canhão, Fonseca: "Early arthritis induces disturbances at bone nanostructural level reflected in decreased tissue hardness in an animal model of arthritis." in: PLoS ONE, Vol. 13, Issue 1, pp. e0190920, (2018) (PubMed).

You, Zu, Liu, Kong, Song, Wei, Zhou, Wang, Yan: "Synovial fibroblast-targeting liposomes encapsulating an NF-κB-blocking peptide ameliorates zymosan-induced synovial inflammation." in: Journal of cellular and molecular medicine, Vol. 22, Issue 4, pp. 2449-2457, (2018) (PubMed).

Gao, Jing, Zhang, Wu et al.: "Pterostilbene protects against acute renal ischemia reperfusion injury and inhibits oxidative stress, inducible nitric oxide synthase expression and inflammation in rats via the Toll-like receptor ..." in: Experimental and therapeutic medicine, Vol. 15, Issue 1, pp. 1029-1035, (2018) (PubMed).

Xu, Qin, Hu, Mu, Zhu, Lu, Luo: "Lentivirus-mediated overexpression of OTULIN ameliorates microglia activation and neuroinflammation by depressing the activation of the NF-κB signaling pathway in cerebral ischemia/reperfusion rats." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 83, (2018) (PubMed).

Chen, Chen, Fan, Wu, Yang, Fang, Fu, Li et al.: "Omega-3 polyunsaturated fatty acid attenuates the inflammatory response by modulating microglia polarization through SIRT1-mediated deacetylation of the HMGB1/NF-κB pathway following experimental ..." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 116, (2018) (PubMed).

Chen, Ye, Chen, Shi, Wu, Lin, Lin, Li, Fu, Li: "Valproic acid attenuates traumatic spinal cord injury-induced inflammation via STAT1 and NF-κB pathway dependent of HDAC3." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 150, (2018) (PubMed).

Zhao, Han: "Dendrobium Officinale Kimura et Migo Ameliorates Insulin Resistance in Rats with Diabetic Nephropathy." in: Medical science monitor basic research, Vol. 24, pp. 84-92, (2018) (PubMed).

Zou, Huang, Lu, Fang, Wang, Zhao, Jia, Xu, Wang, Wang, Dong: "The effects of Jiao-Tai-Wan on sleep, inflammation and insulin resistance in obesity-resistant rats with chronic partial sleep deprivation." in: BMC complementary and alternative medicine, Vol. 17, Issue 1, pp. 165, (2017) (PubMed).

Chen, Huang, Tu, Lu, Xie, Zhao, Wu, Shi, Fang, Wei, Zhang, Lu: "NAMPT inhibitor protects ischemic neuronal injury in rat brain via anti-neuroinflammation." in: Neuroscience, Vol. 356, pp. 193-206, (2017) (PubMed).

Ye, Shen, Huang, Zhang, Xiao: "Anti-fatigue activity of sea cucumber peptides prepared from Stichopus japonicus in an endurance swimming rat model." in: Journal of the science of food and agriculture, Vol. 97, Issue 13, pp. 4548-4556, (2017) (PubMed).

Details zu IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Andere Bezeichnung: IL6

Hintergrund:

Background: Interleukin-6 (IL-6) is a protein that in humans is encoded by the IL6 gene. Interleukin-6 (IL-6) is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. IL-6 is a pleiotropic cytokine that plays a critical role in bone resorption. IL-6 has been suggested to stimulate the HPA axis during immune activation independent of the input of hypothalamic corticotropin-releasing hormone (CRH). Interleukin-6 (IL-6) is an immunoregulatory cytokine that activates a cell-surface signaling assembly composed of IL-6, the IL-6 alpha-receptor (IL-6Ralpha), and the shared signaling receptor gp130. The standard product used in this kit is recombinant rat IL-6, consisting of 188 amino acids with the molecular mass of 21.8 kDa.

Gene Full Name: interleukin 6

Gen-ID: 24498

UniProt: P20607

Pathways: TLR Signalweg, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagie, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome