IL-6 ELISA Kit

Produktnummer ABIN411303

Das Human IL-6 ELISA Kit (ABIN411303) ist ein Colorimetric ELISA Kit zur Detektion von Human IL-6. Das Kit wurde in 147 Publikationen zitiert.

Kurzübersicht für IL-6 ELISA Kit (ABIN411303)

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Bindungsspezifität: AA 29-212

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 4.69 pg/mL - 300 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA), Plasma (citrate)

Produktdetails IL-6 ELISA Kit

Untere Nachweisgrenze: 4.69 pg/mL

Verwendungszweck: Sandwich Human IL-6/Interleukin-6 ELISA Kit to quantitate Human IL6 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: P29-M212

Capture antibody and Detection antibody: monoclonal antibody from mouse, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 0.3 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 6.2%, 4.4%, 7.4%
Inter-Assay Precision (CV%): 7.2%, 4.5%, 8.6%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für IL-6 ELISA Kit (ABIN411303)

Gao, Huang, Zhao, Hu, Li, Guo, Zhao, Lu: "LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling." in: Oncotarget, Vol. 7, Issue 39, pp. 63981-63994, (2018) (PubMed).

Khan, Haseeb, Ansari, Haqqi: "A wogonin-rich-fraction of Scutellaria baicalensis root extract exerts chondroprotective effects by suppressing IL-1β-induced activation of AP-1 in human OA chondrocytes." in: Scientific reports, Vol. 7, pp. 43789, (2018) (PubMed).

Ma, Sun, Zhang, Zhang, Yao: "Proinflammatory effects of S100A8/A9 via TLR4 and RAGE signaling pathways in BV-2 microglial cells." in: International journal of molecular medicine, Vol. 40, Issue 1, pp. 31-38, (2018) (PubMed).

He, Le, Frediani, Winterberg, Jin, Liverman, Hernandez, Cleeton, Vos: "Cardiometabolic risks vary by weight status in pediatric kidney and liver transplant recipients: A cross-sectional, single-center study in the USA." in: Pediatric transplantation, Vol. 21, Issue 6, (2018) (PubMed).

Ünver, Deveci, Kırkıl, Telo, Kaman, Kuluöztürk: "Serum Heat Shock Protein Levels and the Relationship of Heat Shock Proteins with Various Parameters in Chronic Obstructive Pulmonary Disease Patients." in: Turkish thoracic journal, Vol. 17, Issue 4, pp. 153-159, (2018) (PubMed).

Qin, Yan, Wang, Xu, Wang, Zhu, Shi, Li, Zhang, Chen: "Cancer-associated Fibroblast-derived IL-6 Promotes Head and Neck Cancer Progression via the Osteopontin-NF-kappa B Signaling Pathway." in: Theranostics, Vol. 8, Issue 4, pp. 921-940, (2018) (PubMed).

Wen, Gan, Li, Sun, Xiong, Xia: "Safety of Low-calcium Dialysate and its Effects on Coronary Artery Calcification in Patients Undergoing Maintenance Hemodialysis." in: Scientific reports, Vol. 8, Issue 1, pp. 5941, (2018) (PubMed).

Ma, Zhang, Zhao, Wang, Lin, Ma: "Expression of autophagy-related genes in cerebrospinal fluid of patients with tuberculous meningitis." in: Experimental and therapeutic medicine, Vol. 15, Issue 6, pp. 4671-4676, (2018) (PubMed).

Fallah, Feizi, Hashemipour, Kelishadi: "Effect of fermented camel milk on glucose metabolism, insulin resistance, and inflammatory biomarkers of adolescents with metabolic syndrome: A double-blind, randomized, crossover trial." in: Journal of research in medical sciences : the official journal of Isfahan University of Medical Sciences, Vol. 23, pp. 32, (2018) (PubMed).

Sağ, Sağ, Tekeoğlu, Kamanli, Nas, Harman: "The Relationship Between Serum Pentraxine 3 Levels and Hematological Markers in Patients With Rheumatoid Arthritis." in: Archives of rheumatology, Vol. 33, Issue 1, pp. 39-44, (2018) (PubMed).

Csősz, Lábiscsák, Kalló, Márkus, Emri, Szabó, Tar, Tőzsér, Kiss, Márton: "Proteomics investigation of OSCC-specific salivary biomarkers in a Hungarian population highlights the importance of identification of population-tailored biomarkers." in: PLoS ONE, Vol. 12, Issue 5, pp. e0177282, (2017) (PubMed).

Wang, Xie, Yang, Zhang, Wang, Wu, Shen, Xie: "Sulfated Cyclocarya paliurus polysaccharides markedly attenuates inflammation and oxidative damage in lipopolysaccharide-treated macrophage cells and mice." in: Scientific reports, Vol. 7, pp. 40402, (2017) (PubMed).

Ballerini, Diomede, Petragnani, Cicchitti, Merciaro, Cavalcanti, Trubiani: "Conditioned medium from relapsing-remitting multiple sclerosis patients reduces the expression and release of inflammatory cytokines induced by LPS-gingivalis in THP-1 and MO3.13 cell lines." in: Cytokine, Vol. 96, pp. 261-272, (2017) (PubMed).

Jian, Dai, Hu, Yao, Zheng, Zhu: "ox-LDL increases microRNA-29a transcription through upregulating YY1 and STAT1 in macrophages." in: Cell biology international, Vol. 41, Issue 9, pp. 1001-1011, (2017) (PubMed).

Bai, Wei, Wu, Wei, Wang, Bai: "C/EBP β Mediates Endoplasmic Reticulum Stress Regulated Inflammatory Response and Extracellular Matrix Degradation in LPS-Stimulated Human Periodontal Ligament Cells." in: International journal of molecular sciences, Vol. 17, Issue 3, pp. 385, (2017) (PubMed).

Wang, Lu, Zhang, Du, Tang, Wang, Lu, Hu: "GEN-27, a Newly Synthetic Isoflavonoid, Inhibits the Proliferation of Colon Cancer Cells in Inflammation Microenvironment by Suppressing NF-κB Pathway." in: Mediators of inflammation, Vol. 2016, pp. 2853040, (2017) (PubMed).

Alper, Erdogan, Erdogan, Bozan, Can: "Associations of Trauma Severity with Mean Platelet Volume and Levels of Systemic Inflammatory Markers (IL1β, IL6, TNFα, and CRP)." in: Mediators of inflammation, Vol. 2016, pp. 9894716, (2017) (PubMed).

Bao, Li, Luo, Tang, Lv, Li, Zhou: "NF-κB-Regulated miR-99a Modulates Endothelial Cell Inflammation." in: Mediators of inflammation, Vol. 2016, pp. 5308170, (2017) (PubMed).

Guo, Yu, Chen, Wu, Peng, Guo, Zhang: "miR-145 mediated the role of aspirin in resisting VSMCs proliferation and anti-inflammation through CD40." in: Journal of translational medicine, Vol. 14, Issue 1, pp. 211, (2017) (PubMed).

Irtegun, Pektanc, Akkurt, Bozkurt, Turkcu, Kalkanli-Tas: "Pharmacological Inactivation of Src Family Kinases Inhibits LPS-Induced TNF-α Production in PBMC of Patients with Behçet's Disease." in: Mediators of inflammation, Vol. 2016, pp. 5414369, (2017) (PubMed).

Details zu IL-6

Target: IL-6 (IL6) (Interleukin 6 (IL6))

Andere Bezeichnung: IL6

Hintergrund:

Background: The human interferon-beta 2 gene (IFNB2) product is identical to that for the B-cell stimulation factor-2 (BSF-2), the hybridoma growth factor (HGF) (interleukin-6), and the hepatocyte stimulating factor (HSF). Proteins derived from this gene mediate the plasma protein response to tissue injury (acute-phase response) and regulate the growth and differentiation of both B and T cells. Interleukin-6 (IL6) has come to be regarded as a potential osteoporotic factor because it has stimulatory effects on cells of the osteoclast lineage, and, thus, may play a role in the pathogenesis of bone loss associated with estrogen deficiency. IL-6 has many roles essential to the regulation of the immune response, hematopoiesis, and bone resorption. It is involved not only in the hepatic acute phase response but also in adipose tissue metabolism, lipoprotein lipase activity, and hepatic triglyceride secretion. Overproduction of IL-6, a proinflammatory cytokine, is associated with a spectrum of age-related conditions including cardiovascular disease, osteoporosis, arthritis, type 2 diabetes, certain cancers, periodontal disease, frailty, and functional decline. BSF-2 is a novel interleukin consisting of 184 amino acids. The standard product used in this kit is recombinant human IL-6, consisting of 184 amino acids with the molecular mass of 20.3 kDa.

Gene Full Name: interleukin 6

Gen-ID: 3569

UniProt: P05231

Pathways: TLR Signalweg, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagie, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome