C-JUN ELISA Kit

Produktnummer ABIN7830499

Human C-JUN ELISA Kit, Colorimetric Assay für die Quantifizierung von Human C-JUN.

Kurzübersicht für C-JUN ELISA Kit (ABIN7830499)

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Cell ELISA

Applikation: ELISA

Proben: Cell Samples, Suspension Cell Culture

Produktdetails C-JUN ELISA Kit

Verwendungszweck: JUN (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of JUN expression in cultured cells.

Analytische Methode: Qualitative

Kreuzreaktivität: Maus, Ratte

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Uncoated

Protokoll:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: Store the kit at 4°C.

Details zu C-JUN

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Andere Bezeichnung: JUN

Hintergrund:

Synonyms: AP-1|AP1|c-Jun

Gene Description: jun oncogene

Gen-ID: 3725

UniProt: P05412

Pathways: MAPK Signalweg, RTK Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteine