TNFSF13 ELISA Kit

Produktnummer ABIN6951655

Produktdetails TNFSF13 ELISA Kit

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Rat APRIL ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu TNFSF13

Target: TNFSF13 (Tumor Necrosis Factor (Ligand) Superfamily, Member 13 (TNFSF13))

Andere Bezeichnung: APRIL (TNFSF13 Produkte)

Synonyme: APRIL ELISA Kit, CD256 ELISA Kit, TALL-2 ELISA Kit, TALL2 ELISA Kit, TRDL-1 ELISA Kit, ZTNF2 ELISA Kit, 2310026N09Rik ELISA Kit, April ELISA Kit, Tall2 ELISA Kit, Trdl1 ELISA Kit, SLAN ELISA Kit, ANP32B ELISA Kit, april ELISA Kit, tnf13 ELISA Kit, TNLG7B ELISA Kit, TNF superfamily member 13 ELISA Kit, tumor necrosis factor (ligand) superfamily, member 13 ELISA Kit, acidic leucine-rich nuclear phosphoprotein 32 family member B ELISA Kit, TNFSF13 ELISA Kit, Tnfsf13 ELISA Kit, LOC494442 ELISA Kit, tnfsf13 ELISA Kit

Gen-ID: 287437

UniProt: Q5PQL1

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response