SULT1A1 ELISA Kit

Produktnummer ABIN6951477

Produktdetails SULT1A1 ELISA Kit

Target: SULT1A1 (Sulfotransferase Family, Cytosolic, 1A, Phenol-Preferring, Member 1 (SULT1A1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human SULT1A1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu SULT1A1

Target: SULT1A1 (Sulfotransferase Family, Cytosolic, 1A, Phenol-Preferring, Member 1 (SULT1A1))

Andere Bezeichnung: SULT1A1 (SULT1A1 Produkte)

Synonyme: HAST1/HAST2 ELISA Kit, P-PST ELISA Kit, PST ELISA Kit, ST1A1 ELISA Kit, ST1A3 ELISA Kit, STP ELISA Kit, STP1 ELISA Kit, TSPST1 ELISA Kit, AI266890 ELISA Kit, Stp ELISA Kit, Stp1 ELISA Kit, ASTIV ELISA Kit, Mx-ST ELISA Kit, PST-1 ELISA Kit, St1a1 ELISA Kit, Stm ELISA Kit, Sult1a3 ELISA Kit, pst ELISA Kit, st1a3 ELISA Kit, stp ELISA Kit, stp1 ELISA Kit, tspst1 ELISA Kit, cSULT1A1 ELISA Kit, SULT1A2 ELISA Kit, SULT1A1 ELISA Kit, SIAT8-D ELISA Kit, ST8SiaIV ELISA Kit, Siat8d ELISA Kit, PST1 ELISA Kit, SIAT8D ELISA Kit, ST8SIA-IV ELISA Kit, sulfotransferase family 1A member 1 ELISA Kit, sulfotransferase family 1A, phenol-preferring, member 1 ELISA Kit, sulfotransferase family, cytosolic, 1A, phenol-preferring, member 1 ELISA Kit, sulfotransferase family 1A member 1 S homeolog ELISA Kit, sulfotransferase 1A1 ELISA Kit, ST8 alpha-N-acetyl-neuraminide alpha-2,8-sialyltransferase 4 ELISA Kit, SULT1A1 ELISA Kit, Sult1a1 ELISA Kit, sult1a1 ELISA Kit, sult1a1.S ELISA Kit, LOC704658 ELISA Kit, LOC709318 ELISA Kit, St8sia4 ELISA Kit, ST8SIA4 ELISA Kit, LOC100064221 ELISA Kit

Gen-ID: 6817

UniProt: P50225