S100A8 ELISA Kit

Produktnummer ABIN6951104

Produktdetails S100A8 ELISA Kit

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse S100 A8 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Haltbarkeit: 6 months

Details zu S100A8

Target: S100A8 (S100 Calcium Binding Protein A8 (S100A8))

Andere Bezeichnung: S100 A8 (S100A8 Produkte)

Synonyme: 60B8AG ELISA Kit, CAGA ELISA Kit, CFAG ELISA Kit, CGLA ELISA Kit, CP-10 ELISA Kit, L1Ag ELISA Kit, MA387 ELISA Kit, MIF ELISA Kit, MRP8 ELISA Kit, NIF ELISA Kit, P8 ELISA Kit, Mrp8 ELISA Kit, S100A8 ELISA Kit, 60B8Ag ELISA Kit, AI323541 ELISA Kit, B8Ag ELISA Kit, CFAg ELISA Kit, Caga ELISA Kit, p8 ELISA Kit, MRP-8 ELISA Kit, S100 calcium binding protein A8 ELISA Kit, S100 calcium binding protein A8 (calgranulin A) ELISA Kit, S100A8 ELISA Kit, S100a8 ELISA Kit

Gen-ID: 20201

UniProt: P27005

Pathways: Transition Metal Ion Homeostasis, Positive Regulation of Endopeptidase Activity, S100 Proteine