FCGR1A ELISA Kit

Produktnummer ABIN6730653

Produktdetails FCGR1A ELISA Kit

Target: FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Mouse Fc gamma RI/CD64 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Mouse Fc gamma RI.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu FCGR1A

Target: FCGR1A (Fc Fragment of IgG, High Affinity Ia, Receptor (CD64) (FCGR1A))

Andere Bezeichnung: Fc gamma RI (CD64 (FCGR1A Produkte)

Synonyme: CD64 ELISA Kit, CD64A ELISA Kit, FCRI ELISA Kit, IGFR1 ELISA Kit, FcgammaRI ELISA Kit, Fcgr1 ELISA Kit, Fcgr1b ELISA Kit, AI323638 ELISA Kit, AV092959 ELISA Kit, IGGHAFC ELISA Kit, FCGR1A ELISA Kit, Fc fragment of IgG receptor Ia ELISA Kit, Fc receptor, IgG, high affinity I ELISA Kit, FCGR1A ELISA Kit, Fcgr1a ELISA Kit, Fcgr1 ELISA Kit

UniProt: P26151

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process