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Activin A Receptor Type IB/ALK-4 ELISA Kit
ACVR1B
Reaktivität: Maus
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6730326
Produktdetails Activin A Receptor Type IB/ALK-4 ELISA Kit
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Target
Alle Activin A Receptor Type IB/ALK-4 (ACVR1B) ELISA Kits anzeigen
Activin A Receptor Type IB/ALK-4 (ACVR1B)
(Activin A Receptor, Type IB (ACVR1B))
Reaktivität
Alle Reaktivitäten für Activin A Receptor Type IB/ALK-4 ELISA Kits
Maus
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Mouse Activin RIB/ALK-4 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes Mouse Activin RIB.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 µl to 1 µl volumes Adjustable 1-25 µl pipettes for reagent preparation 100 µl and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Discover our top product ACVR1B ELISA Kit
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Anwendungsinformationen
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Applikationshinweise
Optimal working dilution should be determined by the investigator.
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 µl of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4°C. Add 100 µl of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 µl of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 µl of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 µl of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu Activin A Receptor Type IB/ALK-4
(ausblenden)
Target
Alle Activin A Receptor Type IB/ALK-4 (ACVR1B) ELISA Kits anzeigen
Activin A Receptor Type IB/ALK-4 (ACVR1B)
(Activin A Receptor, Type IB (ACVR1B))
Andere Bezeichnung
Activin RIB (ALK-4 (ACVR1B Produkte )
Synonyme
ACVR1B ELISA Kit, alk4 ELISA Kit, skr2 ELISA Kit, alk-4 ELISA Kit, xalk4 ELISA Kit, actrib ELISA Kit, acvrlk4 ELISA Kit, DKFZp469A1614 ELISA Kit, 6820432J04 ELISA Kit, ActR-IB ELISA Kit, ActRIB ELISA Kit, Acvrlk4 ELISA Kit, Alk4 ELISA Kit, SKR2 ELISA Kit, ACTRIB ELISA Kit, ACVRLK4 ELISA Kit, ALK4 ELISA Kit, Skr2 ELISA Kit, axis ELISA Kit, taram-a ELISA Kit, tarama ELISA Kit, wu:fb75f01 ELISA Kit, activin A receptor type 1B ELISA Kit, Activin receptor type-1B ELISA Kit, activin A receptor, type IB ELISA Kit, activin A receptor, type 1B ELISA Kit, activin A receptor type 1Ba ELISA Kit, ACVR1B ELISA Kit, acvr1b ELISA Kit, acv1b ELISA Kit, Acvr1b ELISA Kit, acvr1ba ELISA Kit
UniProt
Q61271
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