COL18A1 ELISA Kit

Produktnummer ABIN6720340

Produktdetails COL18A1 ELISA Kit

Target: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 156 pg/mL - 10000 pg/mL

Untere Nachweisgrenze: 156 pg/mL

Applikation: ELISA

Verwendungszweck: Sandwich ELISA for Quantitative Detection of Antigen

Proben: Breast Milk, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytische Methode: Quantitative

Produktmerkmale:

Synonyms: Alpha 1 collagen type 18 (XVIII)(COL18A1), Alpha 1 type XVIII collagen, Antiangiogenic agent, COL18A1, Collagen alpha 1(XVIII) chain, Collagen type XVIII alpha 1, FLJ27325, FLJ34914, KNO, KNO1, MGC74745, Multi functional protein MFP, OTTMUSP00000023764, OTTMUSP00000023766, Procollagen type XVIII alpha 1

Background: Endostatin is a naturally-occurring 20- kDa C-terminal fragment derived from type XVIII collagen. It is reported to serve as an anti-angiogenic agent, similar to angiostatin and thrombospondin. And it is produced by proteolytic cleavage of collagen XVIII, a member of the multiplexin family that is characterized by interruptions in the triple helix creating multiple domains, by proteases such as cathepsins. Using a genomic clone as a probe for fluorescence in situ hybridization, Endostatin was mapped the COL18A1 gene to 21q22.3. By immunoprecipitation analysis using membrane fractions of human mammary epithelial cells, It showed that endostatin specifically bound to cell surface nucleolin with high affinity. Blockage of nucleolin with neutralizing antibody or knockdown of nucleolin by RNA interference countered the antiendothelial activity of endostatin and abrogated its antiangiogenic and antitumor activity in vivo.

Gene Name: COL18A1

Production: Natural and recombinant human Endostatin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: H1154-S1419

Anwendungsinformationen

Applikationshinweise:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Endostatin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or human milk to each empty well. It is recommended that each human Endostatin standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10000pg/mL

Probenmenge: 100 μL

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: RT,4 °C,-20 °C

Informationen zur Lagerung: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Details zu COL18A1

Target: COL18A1 (Collagen, Type XVIII, alpha 1 (COL18A1))

Andere Bezeichnung: Endostatin (COL18A1 Produkte)

Synonyme: KNO ELISA Kit, KNO1 ELISA Kit, KS ELISA Kit, cb371 ELISA Kit, cb529 ELISA Kit, fc96d06 ELISA Kit, sb:cb529 ELISA Kit, wu:fb47h10 ELISA Kit, wu:fb77g12 ELISA Kit, wu:fc50f04 ELISA Kit, wu:fc96d06 ELISA Kit, wu:fk02h12 ELISA Kit, kno ELISA Kit, kno1 ELISA Kit, collagen type XVIII alpha 1 chain ELISA Kit, collagen type XVIII alpha 1 chain a ELISA Kit, collagen, type XVIII, alpha 1 ELISA Kit, collagen type XVIII alpha 1 ELISA Kit, COL18A1 ELISA Kit, col18a1a ELISA Kit, Col18a1 ELISA Kit, col18a1 ELISA Kit

Substanzklasse: Chemical

Gen-ID: 80781

NCBI Accession: NP_085059

UniProt: P39060