Inhibitory Subunit of NF-KappaB epsilon ELISA Kit
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
Produktnummer ABIN6244347
-
-
Target
-
Inhibitory Subunit of NF-KappaB epsilon
-
Reaktivität
-
Human
-
Nachweismethode
-
Colorimetric
-
Methodentyp
-
Sandwich ELISA
-
Applikation
-
ELISA
-
Verwendungszweck
-
Custom Human IkB-epsilon/NF-kappa-BIE ELISA Kit.
-
Proben
-
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
-
Analytische Methode
-
Quantitative
-
Spezifität
-
This ELISA antibody pair recognizes Human IkB-epsilon.
-
Produktmerkmale
-
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
-
Bestandteile
-
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
-
Benötigtes Material
-
- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
-
-
-
Applikationshinweise
-
Optimal working dilution should be determined by the investigator.
-
Plattentyp
-
Pre-coated
-
Protokoll
-
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
-
Beschränkungen
-
Nur für Forschungszwecke einsetzbar
-
-
Haltbarkeit
-
6 months
-
-
Target
-
Inhibitory Subunit of NF-KappaB epsilon
-
Andere Bezeichnung
-
IkB-epsilon (NF-kappa-BIE)
-
Synonyme
-
IKBE ELISA Kit, NFKB inhibitor epsilon ELISA Kit, nuclear factor of kappa light polypeptide gene enhancer in B cells inhibitor, epsilon ELISA Kit, NFKBIE ELISA Kit, Nfkbie ELISA Kit
-
Gen-ID
-
4794
-
UniProt
-
O00221
-