SLC32A1 ELISA Kit

Produktnummer ABIN5690971

Produktdetails SLC32A1 ELISA Kit

Target: SLC32A1 (Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human VIAAT/SLC32A1 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human VIAAT.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu SLC32A1

Target: SLC32A1 (Solute Carrier Family 32 (GABA Vesicular Transporter), Member 1 (SLC32A1))

Andere Bezeichnung: VIAAT (SLC32A1 (SLC32A1 Produkte)

Synonyme: VGAT ELISA Kit, VIAAT ELISA Kit, vGAT ELISA Kit, Ci-vGAT ELISA Kit, slc32a1 ELISA Kit, viaat ELISA Kit, MGC68938 ELISA Kit, vgat ELISA Kit, xVIAAT ELISA Kit, R75019 ELISA Kit, Viaat ELISA Kit, CG8394 ELISA Kit, CG8394.2 ELISA Kit, Dmel\\CG8394 ELISA Kit, Vgat ELISA Kit, zgc:158324 ELISA Kit, solute carrier family 32 member 1 ELISA Kit, vesicular GABA transporter ELISA Kit, solute carrier family 32 member 1 S homeolog ELISA Kit, vesicular inhibitory amino acid transporter ELISA Kit, Vesicular inhibitory amino acid transporter ELISA Kit, solute carrier family 32 (GABA vesicular transporter), member 1 ELISA Kit, Vesicular GABA Transporter ELISA Kit, SLC32A1 ELISA Kit, vgat ELISA Kit, Bm1_20950 ELISA Kit, slc32a1.S ELISA Kit, slc32a1 ELISA Kit, CpipJ_CPIJ002704 ELISA Kit, viaat ELISA Kit, Slc32a1 ELISA Kit, VGAT ELISA Kit, Tsp_04599 ELISA Kit

Gen-ID: 140679

UniProt: Q9H598

Pathways: Proton Transport