NPPC ELISA Kit

Produktnummer ABIN5690835

Produktdetails NPPC ELISA Kit

Target: NPPC (Natriuretic Peptide Type C (NPPC))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Human C-type Natriuretic Peptide/CNP ELISA Kit.

Proben: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair recognizes Human C-type Natriuretic Peptide.

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu NPPC

Target: NPPC (Natriuretic Peptide Type C (NPPC))

Andere Bezeichnung: C-type Natriuretic Peptide (CNP (NPPC Produkte)

Synonyme: CNPF ELISA Kit, CNPI ELISA Kit, CNPII ELISA Kit, Cnp1 ELISA Kit, CNP ELISA Kit, lbab ELISA Kit, CNP2 ELISA Kit, CNP3 ELISA Kit, cnp ELISA Kit, nppc ELISA Kit, 2',3'-cyclic nucleotide 3' phosphodiesterase ELISA Kit, natriuretic peptide type C ELISA Kit, natriuretic peptide C ELISA Kit, natriuretic peptide C S homeolog ELISA Kit, Cnp ELISA Kit, Nppc ELISA Kit, NPPC ELISA Kit, nppc.S ELISA Kit

Gen-ID: 4880

UniProt: P23582