HADHB ELISA Kit

Produktnummer ABIN5674912

Produktdetails HADHB ELISA Kit

Target: HADHB (Hydroxyacyl-CoA Dehydrogenase/3-Ketoacyl-CoA Thiolase/enoyl-CoA Hydratase (Trifunctional Protein), beta Subunit (HADHB))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0115 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu HADHB

Target: HADHB (Hydroxyacyl-CoA Dehydrogenase/3-Ketoacyl-CoA Thiolase/enoyl-CoA Hydratase (Trifunctional Protein), beta Subunit (HADHB))

Andere Bezeichnung: Hydroxyacyl Coenzyme A Dehydrogenase Beta (HADHb) (HADHB Produkte)

Synonyme: ECHB ELISA Kit, MTPB ELISA Kit, TP-BETA ELISA Kit, thiolase ELISA Kit, fb14g10 ELISA Kit, wu:fb14g10 ELISA Kit, zgc:56274 ELISA Kit, 4930479F15Rik ELISA Kit, Mtpb ELISA Kit, Hadhb ELISA Kit, hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta ELISA Kit, hydroxyacyl-CoA dehydrogenase trifunctional multienzyme complex subunit beta S homeolog ELISA Kit, hydroxyacyl-CoA dehydrogenase/3-ketoacyl-CoA thiolase/enoyl-CoA hydratase (trifunctional protein), beta subunit ELISA Kit, hydroxyacyl-Coenzyme A dehydrogenase/3-ketoacyl-Coenzyme A thiolase/enoyl-Coenzyme A hydratase (trifunctional protein), beta subunit ELISA Kit, HADHB ELISA Kit, hadhb.S ELISA Kit, Hadhb ELISA Kit, hadhb ELISA Kit

Hintergrund: Alternative name: MTPB, Mitochondrial Trifunctional Protein,Beta Subunit, 3-Ketoacyl-Coenzyme A Thiolase/Enoyl-Coenzyme A Hydratase(Trifunctional Protein)Beta Subunit, Beta-ketothiolase

Gen-ID: 231086

UniProt: Q99JY0

Pathways: Monocarboxylic Acid Catabolic Process