C-JUN ELISA Kit

Produktnummer ABIN5674851

Maus C-JUN ELISA Kit, Colorimetric Assay für die Quantifizierung von Maus C-JUN.

Kurzübersicht für C-JUN ELISA Kit (ABIN5674851)

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.391 ng/mL - 25 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Produktdetails C-JUN ELISA Kit

Untere Nachweisgrenze: 0.391 ng/mL

Analytische Methode: Quantitative

Sensitivität: 0172 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu C-JUN

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Andere Bezeichnung: V-Jun Sarcoma Virus 17 Oncogene Homolog (JUN)

Hintergrund: Alternative name: C-Jun, AP1, CJun, p39, Jun Oncogene, Proto-oncogene c-Jun, Activator protein 1, Transcription factor AP-1

Gen-ID: 16476

UniProt: P05627

Pathways: MAPK Signalweg, RTK Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteine