CYP27B1 ELISA Kit

Produktnummer ABIN5674843

Produktdetails CYP27B1 ELISA Kit

Target: CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.78 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.78 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0.29 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu CYP27B1

Target: CYP27B1 (Cytochrome P450, Family 27, Subfamily B, Polypeptide 1 (CYP27B1))

Andere Bezeichnung: Cytochrome P450 27B1 (CYP27B1) (CYP27B1 Produkte)

Synonyme: cp2b ELISA Kit, cyp1 ELISA Kit, cyp1alpha ELISA Kit, cyp27b ELISA Kit, p450c1 ELISA Kit, pddr ELISA Kit, vdd1 ELISA Kit, vddr ELISA Kit, vddri ELISA Kit, vdr ELISA Kit, CP2B ELISA Kit, CYP1 ELISA Kit, CYP1alpha ELISA Kit, CYP27B ELISA Kit, P450c1 ELISA Kit, PDDR ELISA Kit, VDD1 ELISA Kit, VDDR ELISA Kit, VDDRI ELISA Kit, VDR ELISA Kit, Cyp40 ELISA Kit, Cp2b ELISA Kit, Cyp1 ELISA Kit, Cyp27b ELISA Kit, Pddr ELISA Kit, Vdd1 ELISA Kit, Vddr ELISA Kit, VddrI ELISA Kit, Vdr ELISA Kit, cytochrome P450 family 27 subfamily B member 1 ELISA Kit, cytochrome P450, family 27, subfamily B, polypeptide 1 ELISA Kit, cytochrome P450, family 27, subfamily b, polypeptide 1 ELISA Kit, cyp27b1 ELISA Kit, CYP27B1 ELISA Kit, Cyp27b1 ELISA Kit

Hintergrund: Alternative name: VDD1, PDDR, CYP1, P450c1, 25-Hydroxyvitamin D3 1-Alpha-Hydroxylase, Cytochrome P450 Family 27 Subfamily B Polypeptide 1, Calcidiol 1-monooxygenase, VD3 1A hydroxylase

Gen-ID: 1594

UniProt: O15528

Pathways: Metabolism of Steroid Hormones and Vitamin D