TSPO ELISA Kit

Produktnummer ABIN5674815

Produktdetails TSPO ELISA Kit

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.781 ng/mL - 50 ng/mL

Untere Nachweisgrenze: 0.781 ng/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0113 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu TSPO

Target: TSPO (Peripheral-Type Benzodiazepine Receptor (TSPO))

Andere Bezeichnung: Translocator Protein (TSPO) (TSPO Produkte)

Synonyme: LOC732875 ELISA Kit, LOC100136652 ELISA Kit, LOC100280949 ELISA Kit, BZRP ELISA Kit, TSPO ELISA Kit, Bzrp ELISA Kit, IBP ELISA Kit, PBR ELISA Kit, Tspo1 ELISA Kit, MBR ELISA Kit, PTBZR02 ELISA Kit, Ptbzr ELISA Kit, RATPTBZR02 ELISA Kit, BPBS ELISA Kit, DBI ELISA Kit, PBS ELISA Kit, PKBS ELISA Kit, PTBR ELISA Kit, mDRC ELISA Kit, pk18 ELISA Kit, peripheral-type benzodiazepine receptor ELISA Kit, Peripheral-type benzodiazepine receptor ELISA Kit, putative peripheral-type benzodiazepine receptor ELISA Kit, translocator protein ELISA Kit, translocator protein homolog ELISA Kit, hypothetical protein ELISA Kit, LOC732875 ELISA Kit, MAV_3896 ELISA Kit, xccb100_2086 ELISA Kit, Smp_102510 ELISA Kit, Sros_1911 ELISA Kit, tspo ELISA Kit, LOC100280949 ELISA Kit, PGTG_16395 ELISA Kit, TSPO ELISA Kit, Tspo ELISA Kit

Hintergrund: Alternative name: IBP, PKBS, PBR, pk18, mDRC, MBR, BZRP, DBI, PTBR, Mitochondrial benzodiazepine receptor, Peripheral-Type Benzodiazepine Receptor/Recognition Site

Gen-ID: 12257

UniProt: P50637

Pathways: Cellular Response to Molecule of Bacterial Origin