TRPM4 ELISA Kit

Produktnummer ABIN5674771

Produktdetails TRPM4 ELISA Kit

Target: TRPM4 (Transient Receptor Potential Cation Channel, Subfamily M, Member 4 (TRPM4))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0055 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu TRPM4

Target: TRPM4 (Transient Receptor Potential Cation Channel, Subfamily M, Member 4 (TRPM4))

Andere Bezeichnung: Transient Receptor Potential Cation Channel Subfamily M, Member 4 (TRPM4) (TRPM4 Produkte)

Synonyme: PFHB1B ELISA Kit, TRPM4B ELISA Kit, LTrpC-4 ELISA Kit, Mls2s ELISA Kit, 1110030C19Rik ELISA Kit, AW047689 ELISA Kit, LTRPC4 ELISA Kit, TRPM4 ELISA Kit, transient receptor potential cation channel subfamily M member 4 ELISA Kit, transient receptor potential cation channel, subfamily M, member 4 ELISA Kit, TRPM4 ELISA Kit, Trpm4 ELISA Kit

Hintergrund: Alternative name: TRPM4B, Melastatin-4, Calcium-activated non-selective cation channel 1, Long transient receptor potential channel 4

Gen-ID: 54795

UniProt: Q8TD43

Pathways: Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response