TAS1R2 ELISA Kit

Produktnummer ABIN5674684

Produktdetails TAS1R2 ELISA Kit

Target: TAS1R2 (Taste Receptor, Type 1, Member 2 (TAS1R2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.125 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78.125 pg/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 27 pg/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu TAS1R2

Target: TAS1R2 (Taste Receptor, Type 1, Member 2 (TAS1R2))

Andere Bezeichnung: Taste Receptor Type 1 Member 2 (TAS1R2) (TAS1R2 Produkte)

Synonyme: GPR71 ELISA Kit, T1R2 ELISA Kit, TR2 ELISA Kit, Aldh4a1 ELISA Kit, Gpr71 ELISA Kit, Tr2 ELISA Kit, T1r2 ELISA Kit, taste 1 receptor member 2 ELISA Kit, taste receptor, type 1, member 2 ELISA Kit, TAS1R2 ELISA Kit, Tas1r2 ELISA Kit

Hintergrund: Alternative name: TR2, GPR71, T1R2, G Protein-Coupled Receptor 71, Sweet taste receptor T1R2

Gen-ID: 80834

UniProt: Q8TE23