TRPM7 ELISA Kit

Produktnummer ABIN5674682

Produktdetails TRPM7 ELISA Kit

Target: TRPM7 (Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.125 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78.125 pg/mL

Applikation: ELISA

Proben: Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 34 pg/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu TRPM7

Target: TRPM7 (Transient Receptor Potential Cation Channel, Subfamily M, Member 7 (TRPM7))

Andere Bezeichnung: Transient Receptor Potential Cation Channel Subfamily M, Member 7 (TRPM7) (TRPM7 Produkte)

Synonyme: cb495 ELISA Kit, TRPM7 ELISA Kit, chak ELISA Kit, chak1 ELISA Kit, ltrpc7 ELISA Kit, trp-plik ELISA Kit, ALSPDC ELISA Kit, CHAK ELISA Kit, CHAK1 ELISA Kit, LTRPC7 ELISA Kit, TRP-PLIK ELISA Kit, 2310022G15Rik ELISA Kit, 4833414K03Rik ELISA Kit, 5033407O22Rik ELISA Kit, LTrpC-7 ELISA Kit, Ltpr7 ELISA Kit, Ltrpc7 ELISA Kit, TRPPLIK ELISA Kit, Chak ELISA Kit, Trp-plik ELISA Kit, transient receptor potential cation channel subfamily M member 7 ELISA Kit, transient receptor potential cation channel, subfamily M, member 7 ELISA Kit, TRPM7 ELISA Kit, trpm7 ELISA Kit, Trpm7 ELISA Kit

Hintergrund: Alternative name: CHAK, CHAK1, LTRPC7, TRP-PLIK, Channel-kinase 1, Long transient receptor potential channel 7

Gen-ID: 58800

UniProt: Q923J1