COL2A1 ELISA Kit

Produktnummer ABIN5674675

Produktdetails COL2A1 ELISA Kit

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: 0055 ng/mL

Bestandteile: Pre-coated, ready to use 96-well strip plate
Standard (freeze dried)
Standard Diluent
Detection Reagent A
Detection Reagent B
Assay Diluent A
Assay Diluent B
TMB Substhumane
Stop Solution
Wash Buffer(30 x concenthumane)
Plate sealer for 96 wells
Instruction manual

Benötigtes Material: 1. Microplate reader with 450 ± 10nm filter.
2. Precision single or multi-channel pipettes and disposable tips.
3. Eppendorf Tubes for diluting samples.
4. Deionized or distilled water.
5. Absorbent paper for blotting the microtiter plate.
6. Container for Wash Solution.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37℃
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37℃
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37℃
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37℃
8. Add 50µL Stop Solution. Read at 450nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 12 months

Details zu COL2A1

Target: COL2A1 (Collagen, Type II, alpha 1 (COL2A1))

Andere Bezeichnung: CollagenType II Alpha 1 (COL2a1) (COL2A1 Produkte)

Synonyme: ANFH ELISA Kit, AOM ELISA Kit, COL11A3 ELISA Kit, SEDC ELISA Kit, Col2 ELISA Kit, Col2a ELISA Kit, Col2a-1 ELISA Kit, Del1 ELISA Kit, Dmm ELISA Kit, Lpk ELISA Kit, M100856 ELISA Kit, Rgsc856 ELISA Kit, CG2A1A ELISA Kit, COLLII ELISA Kit, col2a1a ELISA Kit, CB11 ELISA Kit, col2a1 ELISA Kit, coll2a1 ELISA Kit, fb38c06 ELISA Kit, fc10c01 ELISA Kit, wu:fb38c06 ELISA Kit, wu:fc10c01 ELISA Kit, collagen type II alpha 1 chain ELISA Kit, collagen, type II, alpha 1 ELISA Kit, collagen, type II, alpha 1 L homeolog ELISA Kit, collagen, type II, alpha 1 S homeolog ELISA Kit, collagen, type II, alpha 1a ELISA Kit, COL2A1 ELISA Kit, Col2a1 ELISA Kit, col2a1 ELISA Kit, col2a1.L ELISA Kit, col2a1.S ELISA Kit, col2a1a ELISA Kit

Hintergrund: Alternative name: COL2-A1, COL2A1, SEDC, Chondrocalcin, Collagen Alpha-1(II)Chain, Primary Osteoarthritis,Spondyloepiphyseal Dysplasia,Congenital

Gen-ID: 12824

UniProt: P28481

Pathways: Sensory Perception of Sound, Growth Factor Binding