TRPM8 ELISA Kit

Produktnummer ABIN5659487

Produktdetails TRPM8 ELISA Kit

Target: TRPM8 (Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Transient Receptor Potential Cation Channel Subfamily M, Member 8 (TRPM8). No significant cross-reactivity or interference between Transient Receptor Potential Cation Channel Subfamily M, Member 8 (TRPM8) and analogues was observed.

Sensitivität: 0.112 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Transient Receptor Potential Cation Channel Subfamily M, Member 8 (TRPM8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Transient Receptor Potential Cation Channel Subfamily M, Member 8 (TRPM8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Transient Receptor Potential Cation Channel Subfamily M, Member 8 (TRPM8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Transient Receptor Potential Cation Channel Subfamily M, Member 8 (TRPM8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Transient Receptor Potential Cation Channel Subfamily M, Member 8 (TRPM8) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Transient Receptor Potential Cation Channel Subfamily M, Member 8 (TRPM8) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu TRPM8

Target: TRPM8 (Transient Receptor Potential Cation Channel, Subfamily M, Member 8 (TRPM8))

Andere Bezeichnung: Transient Receptor Potential Cation Channel Subfamily M, Member 8 (TRPM8 Produkte)

Synonyme: TRPM8 ELISA Kit, trpp8 ELISA Kit, ltrpc6 ELISA Kit, trpm8b ELISA Kit, LTRPC6 ELISA Kit, TRPP8 ELISA Kit, CMR1 ELISA Kit, LTrpC-6 ELISA Kit, Trp-p8 ELISA Kit, transient receptor potential cation channel subfamily M member 8 ELISA Kit, transient receptor potential cation channel subfamily M member 8b L homeolog ELISA Kit, transient receptor potential cation channel, subfamily M, member 8 ELISA Kit, TRPM8 ELISA Kit, trpm8b.L ELISA Kit, Trpm8 ELISA Kit

Hintergrund:

Gene Name: Transient Receptor Potential Cation Channel Subfamily M, Member 8

Gene Aliases: LTRPC6, TRPP8, CMR1, Cold And Menthol Receptor 1, Long transient receptor potential channel 6, Transient receptor potential p8