SLC9A1 ELISA Kit

Produktnummer ABIN5658853

Produktdetails SLC9A1 ELISA Kit

Target: SLC9A1 (Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Sodium/Hydrogen Exchanger 1 (NHE1). No significant cross-reactivity or interference between Sodium/Hydrogen Exchanger 1 (NHE1) and analogues was observed.

Sensitivität: 0.054 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sodium/Hydrogen Exchanger 1 (NHE1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Sodium/Hydrogen Exchanger 1 (NHE1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Sodium/Hydrogen Exchanger 1 (NHE1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Sodium/Hydrogen Exchanger 1 (NHE1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Sodium/Hydrogen Exchanger 1 (NHE1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Sodium/Hydrogen Exchanger 1 (NHE1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu SLC9A1

Target: SLC9A1 (Solute Carrier Family 9, Subfamily A (NHE1, Cation Proton Antiporter 1), Member 1 (SLC9A1))

Andere Bezeichnung: Sodium/Hydrogen Exchanger 1 (SLC9A1 Produkte)

Synonyme: AT-NHX1 ELISA Kit, ATNHX ELISA Kit, ATNHX1 ELISA Kit, NA(+)/H(+) ANTIPORTER ELISA Kit, Na+/H+ exchanger 1 ELISA Kit, T21B4.60 ELISA Kit, T21B4_60 ELISA Kit, AW554487 ELISA Kit, Apnh ELISA Kit, Nhe1 ELISA Kit, swe ELISA Kit, NHE-1 ELISA Kit, NHE1 ELISA Kit, APNH ELISA Kit, Na+/H+ exchanger 1 ELISA Kit, solute carrier family 9 (sodium/hydrogen exchanger), member 1 ELISA Kit, solute carrier family 9 member A1 ELISA Kit, NHX1 ELISA Kit, Slc9a1 ELISA Kit, SLC9A1 ELISA Kit

Hintergrund:

Gene Name: Sodium/Hydrogen Exchanger 1

Gene Aliases: SLC9A1, APNH, Solute Carrier Family 9 Member 1,Sodium/Hydrogen Exchanger, Antiporter,Na+/H+,Amiloride Sensitive, Na(+)/H(+) antiporter, amiloride-sensitive

Pathways: Glycosaminoglycan Metabolic Process, Proton Transport