DROSHA ELISA Kit

Produktnummer ABIN5658503

Produktdetails DROSHA ELISA Kit

Target: DROSHA (Drosha, Ribonuclease Type III (DROSHA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.312 ng/mL - 20 ng/mL

Untere Nachweisgrenze: 0.312 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Ribonuclease III, Nuclear (RNASEN). No significant cross-reactivity or interference between Ribonuclease III, Nuclear (RNASEN) and analogues was observed.

Sensitivität: 0.126 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Ribonuclease III, Nuclear (RNASEN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Ribonuclease III, Nuclear (RNASEN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Ribonuclease III, Nuclear (RNASEN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Ribonuclease III, Nuclear (RNASEN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Ribonuclease III, Nuclear (RNASEN) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Ribonuclease III, Nuclear (RNASEN) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu DROSHA

Target: DROSHA (Drosha, Ribonuclease Type III (DROSHA))

Andere Bezeichnung: Ribonuclease III, Nuclear (DROSHA Produkte)

Synonyme: im:7150667 ELISA Kit, zgc:158612 ELISA Kit, ETOHI2 ELISA Kit, HSA242976 ELISA Kit, RANSE3L ELISA Kit, RN3 ELISA Kit, RNASE3L ELISA Kit, RNASEN ELISA Kit, 1110013A17Rik ELISA Kit, AI874853 ELISA Kit, Etohi2 ELISA Kit, Rn3 ELISA Kit, Rnasen ELISA Kit, RGD1307626 ELISA Kit, ribonuclease type III, nuclear ELISA Kit, Ribonuclease 3 ELISA Kit, ribonuclease 3 ELISA Kit, drosha ribonuclease III ELISA Kit, drosha, ribonuclease type III ELISA Kit, rnasen ELISA Kit, Thicy_0885 ELISA Kit, Sinme_0866 ELISA Kit, Isova_2087 ELISA Kit, Sphch_0770 ELISA Kit, DROSHA ELISA Kit, Drosha ELISA Kit

Hintergrund:

Gene Name: Ribonuclease III, Nuclear

Gene Aliases: RnaseIII, RNASE3L, Rnase-III, Etohi2, DROSHA, RN3, RNASE3-L, Ribonuclease Type III, Double-Stranded RNA-Specific Endoribonuclease

UniProt: Q9NRR4

Pathways: Regulatorische RNA Pathways