MYL2 ELISA Kit

Produktnummer ABIN5657104

Produktdetails MYL2 ELISA Kit

Target: MYL2 (Myosin, Light Chain 2, Regulatory, Cardiac, Slow (MYL2))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8 pg/mL - 500 pg/mL

Untere Nachweisgrenze: 7.8 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Myosin Light Chain 2, Regulatory, Cardiac (MYL2). No significant cross-reactivity or interference between Myosin Light Chain 2, Regulatory, Cardiac (MYL2) and analogues was observed.

Sensitivität: 3.1 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Myosin Light Chain 2, Regulatory, Cardiac (MYL2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Myosin Light Chain 2, Regulatory, Cardiac (MYL2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Myosin Light Chain 2, Regulatory, Cardiac (MYL2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Myosin Light Chain 2, Regulatory, Cardiac (MYL2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Myosin Light Chain 2, Regulatory, Cardiac (MYL2) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Myosin Light Chain 2, Regulatory, Cardiac (MYL2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu MYL2

Target: MYL2 (Myosin, Light Chain 2, Regulatory, Cardiac, Slow (MYL2))

Andere Bezeichnung: Myosin Light Chain 2, Regulatory, Cardiac (MYL2 Produkte)

Synonyme: CMH10 ELISA Kit, MLC2 ELISA Kit, 2410014J02Rik ELISA Kit, MLC-2 ELISA Kit, Mlc2 ELISA Kit, MLC-2v ELISA Kit, Mlc2v ELISA Kit, Mylpc ELISA Kit, Mlc-2 ELISA Kit, myl2 ELISA Kit, zgc:136848 ELISA Kit, XMLC2 ELISA Kit, cmh10 ELISA Kit, mlc2 ELISA Kit, G2 ELISA Kit, MLC-2A ELISA Kit, MLC2V ELISA Kit, myosin light chain 2 ELISA Kit, myosin light chain, phosphorylatable, fast skeletal muscle ELISA Kit, myosin, light polypeptide 2, regulatory, cardiac, slow ELISA Kit, myosin, light chain 2b, regulatory, cardiac, slow ELISA Kit, myosin light chain 2 S homeolog ELISA Kit, myosin, light chain 2, regulatory, cardiac, slow ELISA Kit, MYL2 ELISA Kit, Mylpf ELISA Kit, Myl2 ELISA Kit, myl2b ELISA Kit, myl2.S ELISA Kit

Hintergrund:

Gene Name: Myosin Light Chain 2, Regulatory, Cardiac

Gene Aliases: CMH10, MLC2, Myosin,Light Chain 2,Regulatory,Cardiac,Slow, Myosin regulatory light chain 2, ventricular/cardiac muscle isoform

Gen-ID: 4633

UniProt: P10916

Pathways: Myometrial Relaxation and Contraction