KIR3DL1 ELISA Kit
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- Target Alle KIR3DL1 ELISA Kits anzeigen
- KIR3DL1 (Killer Cell Immunoglobulin-Like Receptor, three Domains, Long Cytoplasmic Tail, 1 (KIR3DL1))
- Reaktivität
- Human
- Nachweismethode
- Colorimetric
- Methodentyp
- Sandwich ELISA
- Detektionsbereich
- 0.156 ng/mL - 10 ng/mL
- Untere Nachweisgrenze
- 0.156 ng/mL
- Applikation
- ELISA
- Proben
- Cell Lysate, Tissue Homogenate
- Analytische Methode
- Quantitative
- Spezifität
- This assay has high sensitivity and excellent specificity for detection of Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1). No significant cross-reactivity or interference between Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1) and analogues was observed.
- Sensitivität
- 0.051 ng/mL
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- Kommentare
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The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
- Testdauer
- 3 h
- Plattentyp
- Pre-coated
- Protokoll
- The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Testpräzision
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Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12% - Beschränkungen
- Nur für Forschungszwecke einsetzbar
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- Handhabung
- The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
- Lagerung
- 4 °C,-20 °C
- Informationen zur Lagerung
- -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
- Haltbarkeit
- 4-8 months
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- Target Alle KIR3DL1 ELISA Kits anzeigen
- KIR3DL1 (Killer Cell Immunoglobulin-Like Receptor, three Domains, Long Cytoplasmic Tail, 1 (KIR3DL1))
- Andere Bezeichnung
- Killer Cell Immunoglobulin Like Receptor 3DL1 (KIR3DL1 Produkte)
- Synonyme
- KIR3DL1 ELISA Kit, KIR3DL3 ELISA Kit, CD158E1 ELISA Kit, KIR ELISA Kit, KIR3DL1/S1 ELISA Kit, NKAT3 ELISA Kit, NKB1 ELISA Kit, NKB1B ELISA Kit, Kirl1 ELISA Kit, Kirl2 ELISA Kit, Krl1 ELISA Kit, killer cell immunoglobulin-like receptor, three domains, long cytoplasmic tail, 1 ELISA Kit, uncharacterized LOC100437736 ELISA Kit, killer cell immunoglobulin like receptor, three Ig domains and long cytoplasmic tail 1 ELISA Kit, KIR3DL1 ELISA Kit, LOC100437736 ELISA Kit, Kir3dl1 ELISA Kit
- Hintergrund
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Gene Name: Killer Cell Immunoglobulin Like Receptor 3DL1
Gene Aliases: CD158E, HLA-BW4-specific inhibitory NK cell receptor, CD158 antigen-like family member E, Natural killer-associated transcript 3, p70 NK receptor CL-2/CL-11
- Gen-ID
- 3811
- UniProt
- P43629
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