NR1I3 ELISA Kit

Produktnummer ABIN5653307

Produktdetails NR1I3 ELISA Kit

Target: NR1I3 (Nuclear Receptor Subfamily 1, Group I, Member 3 (NR1I3))

Reaktivität: Ratte

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.156 ng/mL - 10 ng/mL

Untere Nachweisgrenze: 0.156 ng/mL

Applikation: ELISA

Proben: Cell Lysate, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Constitutive Androstane Receptor (CAR). No significant cross-reactivity or interference between Constitutive Androstane Receptor (CAR) and analogues was observed.

Sensitivität: 0.064 ng/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 3 h

Plattentyp: Pre-coated

Protokoll: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Constitutive Androstane Receptor (CAR). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Constitutive Androstane Receptor (CAR). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Constitutive Androstane Receptor (CAR), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Constitutive Androstane Receptor (CAR) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Constitutive Androstane Receptor (CAR) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Constitutive Androstane Receptor (CAR) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Details zu NR1I3

Target: NR1I3 (Nuclear Receptor Subfamily 1, Group I, Member 3 (NR1I3))

Andere Bezeichnung: Constitutive Androstane Receptor (NR1I3 Produkte)

Synonyme: AA209988 ELISA Kit, AI551208 ELISA Kit, CAR ELISA Kit, CAR-beta ELISA Kit, Care2 ELISA Kit, ESTM32 ELISA Kit, MB67 ELISA Kit, CAR1 ELISA Kit, CXR ELISA Kit, nuclear receptor subfamily 1 group I member 3 ELISA Kit, nuclear receptor subfamily 1, group I, member 3 ELISA Kit, NR1I3 ELISA Kit, Nr1i3 ELISA Kit

Hintergrund:

Gene Name: Constitutive Androstane Receptor

Gene Aliases: NR1I3, NR1-I3, CAR1, MB67, Nuclear Receptor Subfamily 1,Group I,Member 3, Constitutive active response, Constitutive activator of retinoid response, Orphan nuclear receptor MB67

Gen-ID: 65035

UniProt: Q9QUS1

Pathways: Nuclear Receptor Transcription Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway