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BHMT ELISA Kit

BHMT Reaktivität: Human Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
Produktnummer ABIN5652217
  • Target Alle BHMT ELISA Kits anzeigen
    BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))
    Reaktivität
    • 2
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.312 ng/mL - 20 ng/mL
    Untere Nachweisgrenze
    0.312 ng/mL
    Applikation
    ELISA
    Proben
    Cell Lysate, Tissue Homogenate
    Analytische Methode
    Quantitative
    Spezifität
    This assay has high sensitivity and excellent specificity for detection of Betaine Homocysteine Methyltransferase (BHMT). No significant cross-reactivity or interference between Betaine Homocysteine Methyltransferase (BHMT) and analogues was observed.
    Sensitivität
    0.124 ng/mL
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  • Kommentare

    The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

    Testdauer
    3 h
    Plattentyp
    Pre-coated
    Protokoll
    The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Betaine Homocysteine Methyltransferase (BHMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Betaine Homocysteine Methyltransferase (BHMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Betaine Homocysteine Methyltransferase (BHMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Betaine Homocysteine Methyltransferase (BHMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Testpräzision
    Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Betaine Homocysteine Methyltransferase (BHMT) were tested 20 times on one plate, respectively
    Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Betaine Homocysteine Methyltransferase (BHMT) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
    Intra-Assay: CV<10%
    Inter-Assay: CV<12%
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Handhabung
    The Stop Solution is acidic. Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.
    Lagerung
    4 °C,-20 °C
    Informationen zur Lagerung
    -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.
    Haltbarkeit
    4-8 months
  • Target Alle BHMT ELISA Kits anzeigen
    BHMT (Betaine--Homocysteine S-Methyltransferase (BHMT))
    Andere Bezeichnung
    Betaine Homocysteine Methyltransferase (BHMT Produkte)
    Synonyme
    BHMT1 ELISA Kit, hm:zehn2153 ELISA Kit, wu:fb53h01 ELISA Kit, wu:fb63c08 ELISA Kit, wu:fj64d01 ELISA Kit, zgc:123027 ELISA Kit, betaine--homocysteine S-methyltransferase ELISA Kit, betaine--homocysteine S-methyltransferase L homeolog ELISA Kit, betaine-homocysteine methyltransferase ELISA Kit, betaine-homocysteine S-methyltransferase ELISA Kit, BHMT ELISA Kit, bhmt.L ELISA Kit, Bhmt ELISA Kit, bhmt ELISA Kit
    Hintergrund

    Gene Name: Betaine Homocysteine Methyltransferase

    Gene Aliases: Betaine-homocysteine S-methyltransferase

    Gen-ID
    635
    UniProt
    Q93088
    Pathways
    Methionine Biosynthetic Process
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