Vasopressin CLIA Kit

Produktnummer ABIN5651860

Produktdetails Vasopressin CLIA Kit

Target: Vasopressin (AVP) (Arginine Vasopressin (AVP))

Reaktivität: Maus

Nachweismethode: Chemiluminescent

Methodentyp: Competition ELISA

Detektionsbereich: 11.72 pg/mL - 3000 pg/mL

Untere Nachweisgrenze: 11.72 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of Antidiuretic Hormone (ADH). No significant cross-reactivity or interference between Antidiuretic Hormone (ADH) and analogues was observed.

Sensitivität: 4.28 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with a monoclonal antibody specific to Antidiuretic Hormone (ADH). A competitive inhibition reaction is launched between biotin labeled Antidiuretic Hormone (ADH) and unlabeled Antidiuretic Hormone (ADH) (Standards or samples) with the pre-coated antibody specific to Antidiuretic Hormone (ADH). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Antidiuretic Hormone (ADH) in the sample. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is reverse proportional to the Antidiuretic Hormone (ADH) level in the sample or standard.

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Antidiuretic Hormone (ADH) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Antidiuretic Hormone (ADH) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: Vasopressin (AVP) (Arginine Vasopressin (AVP))

Andere Bezeichnung: Antidiuretic Hormone (AVP Produkte)

Synonyme: ADH CLIA Kit, ARVP CLIA Kit, AVP-NPII CLIA Kit, AVRP CLIA Kit, VP CLIA Kit, 2610201A18Rik CLIA Kit, Akr1a4 CLIA Kit, Vp CLIA Kit, Vsp CLIA Kit, DI CLIA Kit, Vas CLIA Kit, Adh CLIA Kit, Adh1a CLIA Kit, Adh1c CLIA Kit, adh CLIA Kit, arvp CLIA Kit, avrp CLIA Kit, avp-npii CLIA Kit, copeptin CLIA Kit, vasotocin CLIA Kit, arginine vasopressin CLIA Kit, aldo-keto reductase family 1, member A1 (aldehyde reductase) CLIA Kit, alcohol dehydrogenase 1 (class I) CLIA Kit, AVP CLIA Kit, Akr1a1 CLIA Kit, Avp CLIA Kit, Adh1 CLIA Kit, avp CLIA Kit

Hintergrund:

Gene Name: Antidiuretic Hormone

Gene Aliases: ARVP, AVP, VP, Arginine Vasopressin, Arg-Vasopressin, Antidiuretic Hormone, Diabetes Insipidus, Neurohypophyseal, Argipressin

Pathways: cAMP Metabolic Process