ADAM10 CLIA Kit

Produktnummer ABIN5651304

Produktdetails ADAM10 CLIA Kit

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Reaktivität: Ratte

Nachweismethode: Chemiluminescent

Methodentyp: Sandwich ELISA

Detektionsbereich: 78.12 pg/mL - 5000 pg/mL

Untere Nachweisgrenze: 78.12 pg/mL

Applikation: ELISA

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of A Disintegrin And Metalloprotease 10 (ADAM10). No significant cross-reactivity or interference between A Disintegrin And Metalloprotease 10 (ADAM10) and analogues was observed.

Sensitivität: 3.13 pg/mL

Anwendungsinformationen

Kommentare:

The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5 % within the expiration date under appropriate storage condition. To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Testdauer: 2 - 3 h

Plattentyp: Pre-coated

Protokoll: The microplate provided in this kit has been pre-coated with an antibody specific to A Disintegrin And Metalloprotease 10 (ADAM10). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to A Disintegrin And Metalloprotease 10 (ADAM10). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the A Disintegrin And Metalloprotease 10 (ADAM10) level in the sample or standard.,

Testpräzision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 10 (ADAM10) were tested 20 times on one plate, respectively
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level A Disintegrin And Metalloprotease 10 (ADAM10) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100
Intra-Assay: CV<10%
Inter-Assay: CV<12%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Do not allow to contact skin or eyes. Calibrators, controls and specimen samples should be assayed in duplicate. Once the procedure has been started, all steps should be completed without interruption.

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: -20°C. Bring all reagents to room temperature before beginning test. The kit may be stored at 4°C for immediate use within two days upon arrival. Reseal any unused strips with desiccant pack. Minimize freeze/thaw cycles.

Haltbarkeit: 4-8 months

Antigendetails

Target: ADAM10 (ADAM Metallopeptidase Domain 10 (ADAM10))

Andere Bezeichnung: A Disintegrin And Metalloprotease 10 (ADAM10 Produkte)

Synonyme: ADAM10 CLIA Kit, Adam10 CLIA Kit, adam10 CLIA Kit, wu:fc03d12 CLIA Kit, ad10 CLIA Kit, cd156c CLIA Kit, kuz CLIA Kit, kuzbanian CLIA Kit, madm CLIA Kit, xadam10 CLIA Kit, ADAM-10 CLIA Kit, ADAM 10 CLIA Kit, AD10 CLIA Kit, CD156c CLIA Kit, HsT18717 CLIA Kit, MADM CLIA Kit, 1700031C13Rik CLIA Kit, LOC100219653 CLIA Kit, zgc:64203 CLIA Kit, ADAM metallopeptidase domain 10 CLIA Kit, ADAM metallopeptidase domain 10a CLIA Kit, ADAM metallopeptidase domain 10 S homeolog CLIA Kit, a disintegrin and metallopeptidase domain 10 CLIA Kit, ADAM metallopeptidase domain 10b CLIA Kit, ADAM10 CLIA Kit, Adam10 CLIA Kit, adam10a CLIA Kit, adam10.S CLIA Kit, adam10b CLIA Kit

Hintergrund:

Gene Name: A Disintegrin And Metalloprotease 10

Gene Aliases: CD156-C, CD156c, MADM, Kuz, CDw156, Kuzbanian protein homolog, Mammalian disintegrin-metalloprotease

UniProt: Q10743

Pathways: Notch Signalweg, EGFR Signaling Pathway