NEDD9 ELISA Kit

Produktnummer ABIN5068005

Produktdetails NEDD9 ELISA Kit

Target: NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: The NEDD9 (Enhancer of filamentation 1) ELISA Kit (Human) is a quantitative sandwich assay for the detection of NEDD9 in human serum, plasma, tissue homogenates and other biological fluids.

Proben: Plasma, Serum, Tissue Homogenate

Analytische Methode: Quantitative

Sensitivität: > 0.094 ng/mL

Produktmerkmale: NEDD9 (Enhancer of filamentation 1) ELISA Kit (Human)

Bestandteile:

• *357566A: Microtiter Strips, 8x12 wells.
• *357566B: Standard, 2 vials
• 357566C: Sample/Standard Dilution Buffer, 1x20ml
• 357566D: Antibody-Biotin (Concentrated), 1x120ul
• 357566E: Antibody Dilution Buffer, 1x10ml
• 357566F: Streptavidin-HRP Conjugate (SABC), 1x120ul
• 357566G: SABC Dilution Buffer, 1x10ml
• 357566H: TMB Substrate, 1x10ml
• 357566J: Stop Solution, 1x10ml
• 357566K: Wash Buffer (25X), 1x30ml

Anwendungsinformationen

Plattentyp: Pre-coated

Protokoll: Principle:

• The microtiter plate provided in this kit has been pre-coated with an antibody specific to NEDD9. Standards and samples are added to the appropriate microtiter plate wells followed by a biotin-conjugated antibody specific to NEDD9. Streptavidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain NEDD9, Biotin-conjugated antibody and enzyme-conjugated Streptavidin complex will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of NEDD9 in the sample is then determined by comparing the O.D. of the sample to the standard curve.

Testpräzision: Precision:

• Intra-Assay CV: <8%
• Inter-Assay CV: <10%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C,-20 °C

Informationen zur Lagerung: 4°C/-20°C

Details zu NEDD9

Target: NEDD9 (Neural Precursor Cell Expressed, Developmentally Down-Regulated 9 (NEDD9))

Andere Bezeichnung: NEDD9 (Enhancer of filamentation 1) (NEDD9 Produkte)

Synonyme: NEDD9 ELISA Kit, CAS-L ELISA Kit, CAS2 ELISA Kit, CASL ELISA Kit, CASS2 ELISA Kit, HEF1 ELISA Kit, Cas-L ELISA Kit, CasL ELISA Kit, E230025G09Rik ELISA Kit, neural precursor cell expressed, developmentally down-regulated 9 ELISA Kit, neural precursor cell expressed, developmentally down-regulated gene 9 ELISA Kit, NEDD9 ELISA Kit, Nedd9 ELISA Kit

UniProt: Q14511