Melatonin Sulfate ELISA Kit

Produktnummer ABIN5067888

Produktdetails Melatonin Sulfate ELISA Kit

Target: Melatonin Sulfate (MS)

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Competition ELISA

Applikation: ELISA

Verwendungszweck: Enzyme immunoassay for the quantitative determination of melatonin sulfate in human urine.

Proben: Urine

Analytische Methode: Quantitative

Sensitivität: 1 ng/mL

Produktmerkmale: Melatonin Sulfate, Human (6-Hydroxymelatonin Sulfate, 6-Sulfatoxymelatonin) ELISA Kit

Bestandteile:

• Microtiter Plate, 1x96 wells
• PAB, 1x200ul
• Melatonin Sulfate (HRP), 40x, 1x200ul
• Standard, 0ng/ml, 1x100ul
• Standard, 1.7ng/ml, 1x100ul
• Standard, 5.2ng/ml, 1x100ul
• Standard, 15.6ng/ml, 1x100ul
• Standard, 46.7ng/ml, 1x100ul
• Standard, 140ng/ml, 1x100ul
• 0: Standard, 420ng/ml, 1x100ul
• 1: Low Control 1, 1x100ul
• 2: High Control 2, 1x100ul
• 3: Assay Buffer, 1x60ml
• 4: Wash Buffer, 20x, 1x50ml
• 5: TMB Substrate, 1x12ml
• 6: Stop Solution, 1M O4, 1x12ml

Anwendungsinformationen

Protokoll: Principle:

• Solid phase enzyme-linked immunosorbent assay (ELISA) based on the competition principle. An unknown amount of antigen present in the sample and a fixed amount of enzyme labelled antigen compete for the binding sites of the antibodies coated onto the wells. After incubation the wells are washed to stop the competition reaction. After the substrate reaction the intensity of the developed color is inversely proportional to the amount of the antigen in the sample. Results of samples can be determined directly using the standard curve.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Informationen zur Lagerung: 4°C

Details zu Melatonin Sulfate

Target: Melatonin Sulfate (MS)

Andere Bezeichnung: Melatonin Sulfate (MS Produkte)