Tumor Necrosis Factor a ELISA Kit

Produktnummer ABIN5026953

Produktdetails

Target: Tumor Necrosis Factor a

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 15.6-1000 pg/mL

Untere Nachweisgrenze: 15.6 pg/mL

Applikation: ELISA

Verwendungszweck: This immunoassay kit allows for the in vitro quantitative determination of Human TNFBeta concentrations in serum, plasma and other biological fluids.

Proben: Plasma, Serum

Analytische Methode: Quantitative

Sensitivität: < 9.4 pg/mL

Bestandteile: plate, standard, diluent, antibodies

Benötigtes Material: pipettes, tubes, reader, incubator

Anwendungsinformationen

Probenmenge: 100 μL

Testdauer: 3 - 4 h

Plattentyp: Pre-coated

Protokoll: This assay employs an antibody specific for Anti-human TNFBeta coated on a 96-well plate. Standards and samples are pipetted into the wells and TNFBeta present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human TNFBeta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TNFBeta bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: protect your eyes

Lagerung: 4 °C

Antigendetails

Target: Tumor Necrosis Factor a

Andere Bezeichnung: TNFalpha (Tumor Necrosis Factor Beta)

Synonyme: DIF ELISA Kit, TNF-alpha ELISA Kit, TNFA ELISA Kit, TNFSF2 ELISA Kit, TNFa ELISA Kit, tumor necrosis factor ELISA Kit, TNF ELISA Kit