IL-6 ELISA Kit
Kurzübersicht für IL-6 ELISA Kit (ABIN5026942)
Target
Alle IL-6 (IL6) ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
Proben
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Untere Nachweisgrenze
- 15.6 pg/mL
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Verwendungszweck
- This assay employs an antibody specific for Anti-human IL-6 coated on a 96-well plate.
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Analytische Methode
- Quantitative
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Sensitivität
- 9.4 pg/mL
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Produktmerkmale
- This immunoassay kit allows for the in vitro quantitative determination of Human IL-6 concentrations in serum, plasma. Detection Range: 15.6 - 1000 pg/mL Sensitivity: < 9.4 pg/mL
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Bestandteile
- plate, standard, diluent, antibodies
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Benötigtes Material
- pipettes, tubes, reader, incubator
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Probenmenge
- 100 μL
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Testdauer
- 3 - 4 h
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Plattentyp
- Pre-coated
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Protokoll
- This assay employs an antibody specific for Anti-human IL-6 coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-6 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-6 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-6 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Vorsichtsmaßnahmen
- protect your eyes
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Please refer to the Manual
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- IL-6 (IL6) (Interleukin 6 (IL6))
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Andere Bezeichnung
- IL-6 (Interleukin 6)
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Pathways
- TLR Signalweg, Hormone Transport, Negative Regulation of Hormone Secretion, Myometrial Relaxation and Contraction, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Carbohydrate Metabolic Process, Autophagie, Cell RedoxHomeostasis, Cancer Immune Checkpoints, Inflammasome
Target Alle IL-6 (IL6) ELISA Kits anzeigen
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