IL-1 beta ELISA Kit
Kurzübersicht für IL-1 beta ELISA Kit (ABIN5026930)
Target
Alle IL-1 beta (IL1B) ELISA Kits anzeigenReaktivität
Nachweismethode
Methodentyp
Detektionsbereich
Applikation
Proben
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Untere Nachweisgrenze
- 7.8 pg/mL
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Verwendungszweck
- This assay employs an antibody specific for anti-human IL-1b coated on a 96-well plate.
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Analytische Methode
- Quantitative
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Sensitivität
- 4.69 pg/mL
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Produktmerkmale
- This immunoassay kit allows for the in vitro quantitative determination of Human IL-1Beta concentrations in serum, plasma. Detection Range: 7.8 - 500 pg/mL Sensitivity: < 4.69 pg/mL
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Bestandteile
- plate, standard, diluent, antibodies
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Benötigtes Material
- pipettes, tubes, reader, incubator
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Interleukin 1, beta (IL1B) ELISA Kit
IL1B Reaktivität: Ratte Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reaktivität: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reaktivität: Maus Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reaktivität: Human AA 117-269 Colorimetric Sandwich ELISA 3.9 pg/mL - 250 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (citrate), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B Reaktivität: Ratte AA 117-268 Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B Reaktivität: Kaninchen Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reaktivität: Maus AA 118-269 Colorimetric Sandwich ELISA 12.5 pg/mL - 800 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum
Interleukin 1, beta (IL1B) ELISA KitIL1B Reaktivität: Human Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Cerebrospinal Fluid, Serum, Urine
Interleukin 1, beta (IL1B) ELISA KitIL1B Reaktivität: Fisch Colorimetric Sandwich ELISA 3.12 pg/mL - 200 pg/mL Plasma, Serum, Tissue Homogenate
Interleukin 1, beta (IL1B) ELISA KitIL1B Reaktivität: Huhn Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Probenmenge
- 100 μL
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Testdauer
- 3 - 4 h
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Plattentyp
- Pre-coated
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Protokoll
- This assay employs an antibody specific for anti-human IL-1b coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-1b present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human IL-1Beta antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-1b bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Beschränkungen
- Nur für Forschungszwecke einsetzbar
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Vorsichtsmaßnahmen
- protect your eyes
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Lagerung
- 4 °C
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Informationen zur Lagerung
- Please refer to the Manual
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- IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))
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Andere Bezeichnung
- IL-1 Beta (Interleukin 1 Beta)
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Pathways
- NF-kappaB Signalweg, Interferon-gamma Pathway, TLR Signalweg, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagie, Cancer Immune Checkpoints, Inflammasome
Target Alle IL-1 beta (IL1B) ELISA Kits anzeigen
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