IL-1 beta ELISA Kit

Produktnummer ABIN411294

Das Maus IL-1 beta ELISA Kit (ABIN411294) ist ein Colorimetric ELISA Kit zur Detektion von Maus IL-1 beta. Das Kit wurde in 59 Publikationen zitiert.

Kurzübersicht für IL-1 beta ELISA Kit (ABIN411294)

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Bindungsspezifität: AA 118-269

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 12.5 pg/mL - 800 pg/mL

Applikation: ELISA

Proben: Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Serum

Produktdetails IL-1 beta ELISA Kit

Untere Nachweisgrenze: 12.5 pg/mL

Verwendungszweck: Sandwich Mouse IL-1 Beta/IL-1F2/IL1B ELISA Kit to quantitate Mouse IL1B in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Analytische Methode: Quantitative

Spezifität: Expression system for standard: E.coli
Immunogen sequence: V118-S269

Capture antibody and Detection antibody: polyclonal antibody from goat, polyclonal antibody from goat

Kreuzreaktivität (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivität: < 1 pg/mL

Bestandteile:

1. Pre-coated 96-well strip microplate
2. Standard
3. Biotinylated antibody (100x)
4. Avidin-Biotin-Peroxidase Complex (100x)
5. Sample Diluent
6. Antibody Diluent
7. Avidin-Biotin-Peroxidase Diluent
8. Color Developing Reagent (TMB)
9. Stop Solution
10. Wash Buffer (25x)
11. Adhesive plate sealers

Benötigtes Material: Microplate Reader capable of reading absorbance at 450nm. Incubator. Automated plate washer (optional). Pipettes and pipette tips capable of precisely dispensing 0.5 μL through 1 mL volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples. Deionized or distilled water. 500 mL graduated cylinders. Test tubes for dilution.

Anwendungsinformationen

Applikationshinweise: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: ELISA Kit is based on standard sandwich enzyme-linked immune-sorbent assay technology. An antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells, a biotinylated detection antibody specific for target is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount in sample captured in plate.

Testpräzision: Intra-Assay Precision (CV%): 5.3%, 4.9%, 7.1%
Inter-Assay Precision (CV%): 6.1%, 6.8%, 7.4%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Handhabung: Avoid multiple freeze-thaw cycles.

Lagerung: -20 °C,4 °C

Informationen zur Lagerung: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Haltbarkeit: 12 months

Referenzen für IL-1 beta ELISA Kit (ABIN411294)

Cai, Chen, Zhong, Zhang, Jiang, Xu, Shi: "Synergistic Effect of Compounds from a Chinese Herb: Compatibility and Dose Optimization of Compounds from N-Butanol Extract of Ipomoea stolonifera." in: Scientific reports, Vol. 6, pp. 27014, (2018) (PubMed).

Pang, Wu, Peng, Yang, Kuai, Qin, Cao, Sun, Chen, Vitek, Jiang: "Potential implications of Apolipoprotein E in early brain injury after experimental subarachnoid hemorrhage: Involvement in the modulation of blood-brain barrier integrity." in: Oncotarget, Vol. 7, Issue 35, pp. 56030-56044, (2018) (PubMed).

Gao, Huang, Zhao, Hu, Li, Guo, Zhao, Lu: "LL202 protects against dextran sulfate sodium-induced experimental colitis in mice by inhibiting MAPK/AP-1 signaling." in: Oncotarget, Vol. 7, Issue 39, pp. 63981-63994, (2018) (PubMed).

Liu, Zhang, Wu, Gong: "Foxo3a-dependent Bim transcription protects mice from a high fat diet via inhibition of activation of the NLRP3 inflammasome by facilitating autophagy flux in Kupffer cells." in: Oncotarget, Vol. 8, Issue 21, pp. 34258-34267, (2018) (PubMed).

Hu, Shen, Wang, Zhao: "Liver X Receptor Agonist TO901317 Attenuates Paraquat-Induced Acute Lung Injury through Inhibition of NF-κB and JNK/p38 MAPK Signal Pathways." in: BioMed research international, Vol. 2017, pp. 4652695, (2018) (PubMed).

Zhang, Wu, Xu, He, Yang, Li, Li, Ao, Cheng, Jia: "The cystathionine β-synthase/hydrogen sulfide pathway contributes to microglia-mediated neuroinflammation following cerebral ischemia." in: Brain, behavior, and immunity, Vol. 66, pp. 332-346, (2018) (PubMed).

Feng, Wei, Wang, Fu, Shi, Qu, Fan: "Rhein ameliorates adenomyosis by inhibiting NF-κB and β-Catenin signaling pathway." in: Biomedicine & pharmacotherapy, Vol. 94, pp. 231-237, (2018) (PubMed).

Wang, Dong, Wang, Liu, Sun, Li, Lin, Geng, Jin, Li: "Adolescent escitalopram prevents the effects of maternal separation on depression- and anxiety-like behaviours and regulates the levels of inflammatory cytokines in adult male mice." in: International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, Vol. 62, pp. 37-45, (2018) (PubMed).

Li, Diao, Guo, Huang, Yang, Feng, Yan, Ning, Zheng, Chen, Wu: "VSIG4 inhibits proinflammatory macrophage activation by reprogramming mitochondrial pyruvate metabolism." in: Nature communications, Vol. 8, Issue 1, pp. 1322, (2018) (PubMed).

Du, Chen, Wang, Sun: "Pathway analysis of global gene expression change in dendritic cells induced by the polysaccharide from the roots of Actinidia eriantha." in: Journal of ethnopharmacology, Vol. 214, pp. 141-152, (2018) (PubMed).

Wu, Zhang, Qi, Zhang, Li, Li, Lin, Bai, Liu, Chen, Yang, Xu, Zhang, Yang: "Nicotine promotes atherosclerosis via ROS-NLRP3-mediated endothelial cell pyroptosis." in: Cell death & disease, Vol. 9, Issue 2, pp. 171, (2018) (PubMed).

Zhang, Li, Xu, Xiang, Ma: "Luteolin alleviates NLRP3 inflammasome activation and directs macrophage polarization in lipopolysaccharide-stimulated RAW264.7 cells." in: American journal of translational research, Vol. 10, Issue 1, pp. 265-273, (2018) (PubMed).

Zhang, Feng, Cui, Yang, Zhu et al.: "Pre-B cell leukemia transcription factor 3 induces inflammatory responses in human umbilical vein endothelial cells and murine sepsis via acting a competing endogenous RNA for high mobility group box ..." in: Molecular medicine reports, Vol. 17, Issue 4, pp. 5805-5813, (2018) (PubMed).

Lu, Zhang, Zhang, Zhou, Gao, Liu, Wang, Wu, Li, Hang: "Peroxiredoxin 2 activates microglia by interacting with Toll-like receptor 4 after subarachnoid hemorrhage." in: Journal of neuroinflammation, Vol. 15, Issue 1, pp. 87, (2018) (PubMed).

Xu, Liang, Li, Ma: "Signaling via the CXCR5/ERK pathway is mediated by CXCL13 in mice with breast cancer." in: Oncology letters, Vol. 15, Issue 6, pp. 9293-9298, (2018) (PubMed).

Xianchu, Ming, Xiangbin, Lan: "Grape seed proanthocyanidin extract supplementation affects exhaustive exercise-induced fatigue in mice." in: Food & nutrition research, Vol. 62, (2018) (PubMed).

Song, Pei, Hu, Pan, Xiong, Liu, Wu, Shang, Yao: "Death-associated protein kinase 1 mediates interleukin-1β production through regulating inlfammasome activation in Bv2 microglial cells and mice." in: Scientific reports, Vol. 8, Issue 1, pp. 9930, (2018) (PubMed).

Zhang, Li, Liu, Chen, Gong: "Activation of imidazoline I1 receptor by moxonidine regulates the progression of liver fibrosis in the Nrf2-dependent pathway." in: Biomedicine & pharmacotherapy, Vol. 90, pp. 821-834, (2017) (PubMed).

Yang, Dong, Hu, Chen, Fu, Wang, Xu, Tu: "Liver Kinase B1/AMP-Activated Protein Kinase Pathway Activation Attenuated the Progression of Endotoxemia in the Diabetic Mice." in: Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, Vol. 42, Issue 2, pp. 761-779, (2017) (PubMed).

Jin, Liu, Chen, Xiang, Ding, Li: "Effect of miR-146 targeted HDMCP up-regulation in the pathogenesis of nonalcoholic steatohepatitis." in: PLoS ONE, Vol. 12, Issue 3, pp. e0174218, (2017) (PubMed).

Details zu IL-1 beta

Target: IL-1 beta (IL1B) (Interleukin 1, beta (IL1B))

Andere Bezeichnung: IL1B

Hintergrund:

Background: Interleukin-1beta (IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B (NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

Gene Full Name: interleukin 1 beta

Gen-ID: 16176

UniProt: P10749

Pathways: NF-kappaB Signalweg, Interferon-gamma Pathway, TLR Signalweg, Negative Regulation of Hormone Secretion, Cellular Response to Molecule of Bacterial Origin, Carbohydrate Homeostasis, Glycosaminoglycan Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Autophagie, Cancer Immune Checkpoints, Inflammasome