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GSK3 alpha ELISA Kit

Dieses Human, Ratte und Maus GSK3 alpha ELISA-Kit ist ein Colorimetric ELISA-Kit, das dafür entwickelt wurde, Human, Ratte und Maus GSK3 alpha zu quantifizieren.
Produktnummer ABIN4886117

Kurzübersicht für GSK3 alpha ELISA Kit (ABIN4886117)

Target

Alle GSK3 alpha (GSK3a) ELISA Kits anzeigen
GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

Bindungsspezifität

pSer21, total

Reaktivität

  • 6
  • 5
  • 5
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
  • 1
Human, Ratte, Maus

Nachweismethode

Colorimetric

Methodentyp

Sandwich ELISA

Applikation

ELISA

Proben

Cell Lysate, Tissue Lysate
  • Verwendungszweck

    Human/Mouse/Rat Phospho-GSK3a (S21) and Total GSK3a ELISA Kit. This assay semi-quantitatively measures phophorylated GSK3a (Ser21) and Total GSK3a in lysate samples.

    Analytische Methode

    Semi-Quantitative

    Spezifität

    The antibody pair provided in this kit recognizes human Phospho-GSK3 alpha (pSer21) and total GSK3 alpha.

    Produktmerkmale

    • Simultaneously measure Phosphorylated protein and pan protein in one experiment (for normalization purpose)
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material

    Bestandteile

    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • Anti-Pan Antibody
    • HRP-Conjugated Secondary Antibody
    • Streptavidin-Conjugated HRP
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample

    Benötigtes Material

    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
  • Probenmenge

    100 μL

    Plattentyp

    Pre-coated

    Protokoll

    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.

    Beschränkungen

    Nur für Forschungszwecke einsetzbar
  • Lagerung

    -20 °C

    Informationen zur Lagerung

    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

    Haltbarkeit

    6 months
  • Target Alle GSK3 alpha (GSK3a) ELISA Kits anzeigen

    GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))

    Andere Bezeichnung

    GSK3a

    Gen-ID

    2931

    UniProt

    P49840

    Pathways

    PI3K-Akt Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling, Warburg Effekt
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