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GSK3 alpha ELISA Kit (Glycogen Synthase Kinase 3 alpha) ELISA Kit

GSK3a Reaktivität: Human, Maus pSer21 Colorimetric Cell ELISA Cell Culture Cells
Produktnummer ABIN4881283
$458.70
Zzgl. Versandkosten $45.00
Lieferung in 2 bis 3 Werktagen
  • Target Alle GSK3 alpha (GSK3a) ELISA Kits anzeigen
    GSK3 alpha (GSK3a)
    Bindungsspezifität
    pSer21
    Reaktivität
    • 7
    • 7
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus
    Nachweismethode
    Colorimetric
    Methodentyp
    Cell ELISA
    Applikation
    ELISA
    Verwendungszweck
    Cell-Based Human/Mouse GSK-3a (S21) Phosphorylation ELISA Kit. Suitable for adherent whole cell lines.
    Proben
    Cell Culture Cells
    Analytische Methode
    Semi-Quantitative
    Spezifität
    The antibodies provided in this kit recognizes human and mouse GSK-3 alpha phosphorylated at Serine-21 and total GSK-3 alpha for comparison.
    Produktmerkmale
    • Site and signal pathway-specific
    • In vitro detection of adherent cell culture
    • No sample lysis needed
    • Compatible with a standard ELISA plate reader
    • Faster results than with ELISA
    • Adaptable for high-throughput screening and drug discovery
    Bestandteile
    • uncoated 96-well Microplate
    • Wash Buffer A
    • Wash Buffer B
    • Fixing Solution
    • Quenching Buffer
    • Blocking Buffer
    • Anti-phospho antibody
    • Anti-pan antibody
    • HRP-Conjugated Secondary Antibody
    • TMB One-Step Substrate
    • Stop Solution
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Featured
    Zu unserem meistverkauften GSK3a ELISA Kit
  • Probenmenge
    100 μL
    Plattentyp
    Uncoated
    Protokoll
    1. Seed 10,000-30,000 cells into each well and incubate overnight.
    2. Apply various treatment, inhibitors or activators according to manufacture's instructions.
    3. Add 100 μL of Fixing Solution into each well and incubate for 20 min at RT with shaking.
    4. Add 200 μL of prepared 1X Quenching Buffer and incubate 20 min at RT.
    5. Add 200 μL of Blocking Solution and incubate for 1 h at 37 °C.
    6. Add 50 μL of 1X anti-phospho-protein specific antibody or anti-pan-protein specific antibody to each well and incubate for 2 h at RT.
    7. Add 50 μL of prepared 1X HRP-Anti-Rabbit or Mouse IgG and incubate for 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 6 months from the date of shipment. Avoid repeated freeze-thaw cycles.
    Haltbarkeit
    6 months
  • Target Alle GSK3 alpha (GSK3a) ELISA Kits anzeigen
    GSK3 alpha (GSK3a)
    Andere Bezeichnung
    GSK-3a (GSK3a Produkte)
    Synonyme
    2700086H06Rik, GSK3A, GSK-3[a], GSK-3[b], GSK3, gsk3a, xgsk3, glycogen synthase kinase 3 alpha, glycogen synthase kinase 3 alpha b, glycogen synthase kinase 3 alpha S homeolog, GSK3A, Gsk3a, gsk3a, gsk3ab, gsk3a.S, Bm1_31380
    Gen-ID
    2931
    UniProt
    P49840
    Pathways
    PI3K-Akt Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling
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