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GSK3 alpha ELISA Kit

GSK3a Reaktivität: Human, Maus, Ratte pSer21 Colorimetric Sandwich ELISA Cell Lysate, Tissue Lysate
Produktnummer ABIN4886114
$584.10
Zzgl. Versandkosten $45.00
Lieferung in 2 bis 3 Werktagen
  • Target Alle GSK3 alpha (GSK3a) ELISA Kits anzeigen
    GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))
    Bindungsspezifität
    pSer21
    Reaktivität
    • 7
    • 6
    • 6
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Maus, Ratte
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Applikation
    ELISA
    Verwendungszweck
    Human/Mouse/Rat Phospho-GSK3a (S21) ELISA Kit. This assay semi-quantitatively measures phophorylated GSK3a (Ser21) in lysate samples.
    Proben
    Cell Lysate, Tissue Lysate
    Analytische Methode
    Semi-Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes human Phospho-GSK3 alpha (pSer21).
    Produktmerkmale
    • Rapidly measure phosphorylated protein in lysates
    • Screen numerous different cell lysates without performing a Western Blot analysis
    • Minimal hands-on time, convenient, and non-radioactive material
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Anti-Phospho Antibody
    • HRP-Conjugated Secondary Antibody
    • Assay Diluent
    • TMB One-Step Substrate
    • Stop Solution
    • Lysis Buffer
    • Positive Control Sample
    Benötigtes Material
    • Distilled or deionized water
    • 100 mL and 1 liter graduated cylinders
    • Tubes to prepare sample dilutions
    • Protease and Phosphatase inhibitors
    • Precision pipettes to deliver 2 μL to 1 mL volumes
    • Adjustable 1-25 mL pipettes for reagent preparation
    • Benchtop rocker or shaker
    • Microplate reader capable of measuring absorbance at 450 nm
    Featured
    Zu unserem meistverkauften GSK3a ELISA Kit
  • Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents and samples as instructed in the manual.
    2. Add 100 μL of sample or positive control to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared primary antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
    7. Incubate 1 h at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.
    Haltbarkeit
    6 months
  • Target Alle GSK3 alpha (GSK3a) ELISA Kits anzeigen
    GSK3 alpha (GSK3a) (Glycogen Synthase Kinase 3 alpha (GSK3a))
    Andere Bezeichnung
    GSK3a (GSK3a Produkte)
    Synonyme
    2700086H06Rik, GSK3A, GSK-3[a], GSK-3[b], GSK3, gsk3a, xgsk3, glycogen synthase kinase 3 alpha, glycogen synthase kinase 3 alpha b, glycogen synthase kinase 3 alpha S homeolog, GSK3A, Gsk3a, gsk3a, gsk3ab, gsk3a.S, Bm1_31380
    Gen-ID
    2931
    UniProt
    P49840
    Pathways
    PI3K-Akt Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, EGFR Signaling Pathway, Neurotrophin Signalübertragung, cAMP Metabolic Process, Cellular Glucan Metabolic Process, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, BCR Signaling
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