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SULT2A1 ELISA Kit
SULT2A1
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4884694
Produktdetails SULT2A1 ELISA Kit
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Target
Alle SULT2A1 ELISA Kits anzeigen
SULT2A1
(Sulfotransferase Family, Cytosolic, 2A, Dehydroepiandrosterone (DHEA)-Preferring, Member 1 (SULT2A1))
Reaktivität
Alle Reaktivitäten für SULT2A1 ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human SULT2A1 ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Alternativen
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu SULT2A1
(ausblenden)
Target
Alle SULT2A1 ELISA Kits anzeigen
SULT2A1
(Sulfotransferase Family, Cytosolic, 2A, Dehydroepiandrosterone (DHEA)-Preferring, Member 1 (SULT2A1))
Andere Bezeichnung
SULT2A1 (SULT2A1 Produkte )
Synonyme
dhea-st ELISA Kit, dheas ELISA Kit, st2 ELISA Kit, st2a1 ELISA Kit, st2a3 ELISA Kit, sta ELISA Kit, std ELISA Kit, SULT2A1 ELISA Kit, ST2A1 ELISA Kit, Std ELISA Kit, Sth1 ELISA Kit, mSTa1 ELISA Kit, STa ELISA Kit, Smp-2 ELISA Kit, St2 ELISA Kit, St2a1 ELISA Kit, Sth2 ELISA Kit, DHEA-ST ELISA Kit, DHEAS ELISA Kit, HST ELISA Kit, ST2 ELISA Kit, ST2A3 ELISA Kit, STD ELISA Kit, hSTa ELISA Kit, sulfotransferase family 2A member 1 ELISA Kit, sulfotransferase family 2A member 1 L homeolog ELISA Kit, alcohol sulfotransferase ELISA Kit, sulfotransferase family 2A, dehydroepiandrosterone (DHEA)-preferring, member 1 ELISA Kit, SULT2A1 ELISA Kit, sult2a1.L ELISA Kit, PCC8801_2155 ELISA Kit, Cyan8802_2217 ELISA Kit, NAL212_1203 ELISA Kit, sult2a1 ELISA Kit, Sult2a1 ELISA Kit
Gen-ID
6822
UniProt
Q06520
Pathways
Steroid Hormone Biosynthesis , Regulation of Lipid Metabolism by PPARalpha , Monocarboxylic Acid Catabolic Process
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