SERPINB6 ELISA Kit

Produktnummer ABIN4884484

Produktdetails SERPINB6 ELISA Kit

Target: SERPINB6 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 6 (SERPINB6))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Applikation: ELISA

Verwendungszweck: Custom Human Serpin B6 ELISA Kit.

Proben: Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate

Analytische Methode: Quantitative

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu SERPINB6

Target: SERPINB6 (serpin Peptidase Inhibitor, Clade B (Ovalbumin), Member 6 (SERPINB6))

Andere Bezeichnung: Serpin B6 (SERPINB6 Produkte)

Synonyme: Serpinb6a ELISA Kit, cap ELISA Kit, dfnb91 ELISA Kit, mstp057 ELISA Kit, pi6 ELISA Kit, pti ELISA Kit, serpinb8 ELISA Kit, spi3 ELISA Kit, 4930482L21Rik ELISA Kit, D330015H01Rik ELISA Kit, PI-6 ELISA Kit, Serpinb6 ELISA Kit, Spi3 ELISA Kit, ovalbumin ELISA Kit, CAP ELISA Kit, DFNB91 ELISA Kit, MSTP057 ELISA Kit, PI6 ELISA Kit, PTI ELISA Kit, SPI3 ELISA Kit, serpin family B member 6A ELISA Kit, serpin family B member 6 ELISA Kit, serine (or cysteine) peptidase inhibitor, clade B, member 6a ELISA Kit, serpin peptidase inhibitor, clade B (ovalbumin), member 6 ELISA Kit, Serpinb6a ELISA Kit, serpinb6 ELISA Kit, SERPINB6 ELISA Kit

Gen-ID: 5269

UniProt: P35237