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SEMA6A ELISA Kit
SEMA6A
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4884456
Produktdetails SEMA6A ELISA Kit
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Target
Alle SEMA6A ELISA Kits anzeigen
SEMA6A
(Sema Domain, Transmembrane Domain (TM), and Cytoplasmic Domain, (Semaphorin) 6A (SEMA6A))
Reaktivität
Alle Reaktivitäten für SEMA6A ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human Semaphorin 6A ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes human Semaphorin 6A. Other species not yet determined.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
Alternativen
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu SEMA6A
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Target
Alle SEMA6A ELISA Kits anzeigen
SEMA6A
(Sema Domain, Transmembrane Domain (TM), and Cytoplasmic Domain, (Semaphorin) 6A (SEMA6A))
Andere Bezeichnung
Semaphorin 6A (SEMA6A Produkte )
Synonyme
fj46c12 ELISA Kit, wu:fj46c12 ELISA Kit, zgc:66106 ELISA Kit, SEMA6A ELISA Kit, HT018 ELISA Kit, SEMA ELISA Kit, SEMA6A1 ELISA Kit, SEMAQ ELISA Kit, VIA ELISA Kit, 9330158E07 ELISA Kit, A730020P05Rik ELISA Kit, AI851735 ELISA Kit, Sema6A-1 ELISA Kit, Semaq ELISA Kit, VIa ELISA Kit, b2b997Clo ELISA Kit, sema domain, transmembrane domain (TM), and cytoplasmic domain, (semaphorin) 6A ELISA Kit, semaphorin 6A ELISA Kit, sema6a ELISA Kit, SEMA6A ELISA Kit, Sema6a ELISA Kit
Gen-ID
57556
UniProt
Q9H2E6
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