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ACVR2A ELISA Kit
ACVR2A
Reaktivität: Human
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Produktnummer ABIN4881727
Produktdetails ACVR2A ELISA Kit
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Target
Alle ACVR2A ELISA Kits anzeigen
ACVR2A
(Activin A Receptor, Type IIA (ACVR2A))
Reaktivität
Alle Reaktivitäten für ACVR2A ELISA Kits
Human
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Applikation
ELISA
Verwendungszweck
Custom Human Activin R2A ELISA Kit.
Proben
Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Lysate
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair recognizes human Activin R2A. Other species not yet determined.
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu ACVR2A
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Target
Alle ACVR2A ELISA Kits anzeigen
ACVR2A
(Activin A Receptor, Type IIA (ACVR2A))
Andere Bezeichnung
Activin R2A (ACVR2A Produkte )
Synonyme
ACVR2 ELISA Kit, ACVR2A ELISA Kit, XSTK9 ELISA Kit, actrii ELISA Kit, acvr2 ELISA Kit, ACTRII ELISA Kit, Acvr2 ELISA Kit, rActR-II ELISA Kit, actr2a ELISA Kit, acvr2a ELISA Kit, wu:fa02e10 ELISA Kit, ActrIIa ELISA Kit, TactrII ELISA Kit, activin A receptor type 2A ELISA Kit, activin A receptor type 2A L homeolog ELISA Kit, activin A receptor type 2Aa ELISA Kit, activin receptor IIA ELISA Kit, ACVR2A ELISA Kit, acvr2a ELISA Kit, Acvr2a ELISA Kit, acvr2a.L ELISA Kit, acvr2aa ELISA Kit
Gen-ID
92
UniProt
P27037
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