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2B4 ELISA Kit

CD244 Reaktivität: Human Colorimetric Sandwich ELISA 0.05-12 ng/mL Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN4881695
  • Target Alle 2B4 (CD244) ELISA Kits anzeigen
    2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))
    Reaktivität
    • 6
    • 1
    Human
    Nachweismethode
    Colorimetric
    Methodentyp
    Sandwich ELISA
    Detektionsbereich
    0.05-12 ng/mL
    Untere Nachweisgrenze
    0.05 ng/mL
    Applikation
    ELISA
    Verwendungszweck
    Human 2B4 (CD244) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.
    Proben
    Plasma, Cell Culture Supernatant, Serum
    Analytische Methode
    Quantitative
    Spezifität
    The antibody pair provided in this kit recognizes human 2B4/CD244/SLAMF4.
    Produktmerkmale
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Bestandteile
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Benötigtes Material
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Applikationshinweise
    Recommended Dilution for serum and plasma samples2 fold
    Probenmenge
    100 μL
    Plattentyp
    Pre-coated
    Protokoll
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Beschränkungen
    Nur für Forschungszwecke einsetzbar
  • Lagerung
    -20 °C
    Informationen zur Lagerung
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Haltbarkeit
    6 months
  • Target Alle 2B4 (CD244) ELISA Kits anzeigen
    2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))
    Andere Bezeichnung
    2B4 (CD244 Produkte)
    Synonyme
    CD244 ELISA Kit, 2B4 ELISA Kit, NAIL ELISA Kit, NKR2B4 ELISA Kit, Nmrk ELISA Kit, SLAMF4 ELISA Kit, C9.1 ELISA Kit, F730046O15Rik ELISA Kit, Ly90 ELISA Kit, CD244 molecule ELISA Kit, CD244 natural killer cell receptor 2B4 ELISA Kit, CD244 ELISA Kit, Cd244 ELISA Kit
    Gen-ID
    51744
    UniProt
    Q9BZW8
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