Pancreatic Prohormone Or Pancreatic Polypeptide (ProPPY, ProPNP) and Pancreatic Icosapeptide (PI)
Reaktivität
Human, Maus, Ratte
Nachweismethode
Colorimetric
Methodentyp
Competition ELISA
Detektionsbereich
0.1-1.000 ng/mL
Untere Nachweisgrenze
0.1 ng/mL
Applikation
ELISA
Verwendungszweck
Human/Mouse/Rat ProPPY/PI EIA Kit optimized for serum and cell culture supernatants. Competition-based ELISA on a 96-well strip plate.
Proben
Plasma, Cell Culture Supernatant, Serum
Analytische Methode
Quantitative
Spezifität
This EIA kit is designed to detect human, mouse, and rat pro-pancreatic polypeptide (Pro-PPY) and pancreatic icosapeptide, not active pancreatic polypeptide.
Sensitivität
0.5 ng/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments
Quantitative protein detection
Establishes normal range
The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate
Wash Buffer
Standard Peptide
Assay Diluent(s)
Biotinylated Peptide
HRP-Streptavidin
TMB One-Step Substrate
Stop Solution
Assay Diagram
Positive Control Sample
Capture Antibody
User Manual
Benötigtes Material
Distilled or deionized water
Precision pipettes to deliver 2 μL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
100 mL and 1 liter graduated cylinders
Tubes to prepare standard and sample dilutions
Orbital shaker
Aluminum foil
Saran Wrap
Absorbent paper
Microplate reader capable of measuring absorbance at 450nm
SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Recommended Dilution for serum and plasma samplesHuman: 2X / Mouse: 2X / Rat: 2X
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed.
Add 100 μL detection antibody to each well.
Incubate 1.5 h at RT or O/N at 4 °C.
Add 100 μL standard or sample to each well.
Incubate 2.5 h at RT.
Add 100 μL prepared streptavidin solution.
Incubate 45 min at RT.
Add 100 μL TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL Stop Solution to each well.
Read plate at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Lagerung
-20 °C
Informationen zur Lagerung
Standard, Biotinylated ProPPY peptide, and Positive Control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened Microplate Wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
Haltbarkeit
6 months
Target
Pancreatic Prohormone Or Pancreatic Polypeptide (ProPPY, ProPNP) and Pancreatic Icosapeptide (PI)