ADMA ELISA Kit

Produktnummer ABIN367029

Produktdetails ADMA ELISA Kit

Target: ADMA (Asymmetrical Dimethylarginine (ADMA))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 7.8-500 ng/mL

Untere Nachweisgrenze: 7.8 ng/mL

Applikation: ELISA

Verwendungszweck: For the quantitative determination of endogenic human asymmetrical dimethylarginine (ADMA) concentrations in serum, plasma, tissue homogenates, cell lysates.

Proben: Serum, Plasma, Tissue Homogenate, Cell Lysate

Analytische Methode: Quantitative

Spezifität: This assay has high sensitivity and excellent specificity for detection of human ADMA.

Kreuzreaktivität (Details): Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between the target antigen and all analogues for other species. Therefore, cross reaction may still exist.

Sensitivität: 1.95 ng/mL

Bestandteile:

• Assay plate (12 × 8 coated Microwells)
• Standard (freeze dried)
• Biotin-antibody (100 × concentrate)
• HRP-avidin (100 × concentrate)
• Biotin-antibody Diluent
• HRP-avidin Diluent
• Sample Diluent
• Wash Buffer (25 × concentrate)
• TMB Substrate
• Stop Solution
• Adhesive Strip (for 96 wells)
• Instruction manual

Anwendungsinformationen

Applikationshinweise:

• The supplier is only responsible for the kit itself, but not for the samples consumed during the assay. The user should calculate the possible amount of the samples used in the whole test. Please reserve sufficient samples in advance.
• Samples to be used within 5 days may be stored at 2-8°C, otherwise samples must be stored at -20°C (≤ 1 month) or -80°C (≤ 2 months) to avoid loss of bioactivity and contamination.
• Grossly hemolyzed samples are not suitable for use in this assay.
• If the samples are not indicated in the manual, a preliminary experiment to determine the validity of the kit is necessary.
• Please predict the concentration before assaying. If values for these are not within the range of the standard curve, users must determine the optimal sample dilutions for their particular experiments.
• Tissue or cell extraction samples prepared by chemical lysis buffer may cause unexpected ELISA results due to the impacts of certain chemicals.
• Owing to the possibility of mismatching between antigens from another resource and antibodies used in this supplier's kits (e.g., antibody targets conformational epitope rather than linear epitope), some native or recombinant proteins from other manufacturers may not be recognized by this supplier's products.
• Influenced by factors including cell viability, cell number and cell sampling time, samples from cell culture supernatant may not be recognized by the kit.
• Fresh samples without long time storage are recommended for the test. Otherwise, protein degradation and denaturalization may occur in those samples and finally lead to wrong results.

Kommentare:

Detection wavelength: 450 nm

Information on standard material:
Depending on the antigen to be detected, standards can be either native or recombinant protein. The recombinant proteins are being expressed in CHO cells in most cases. Please inquire for more information. The formulation of auxiliary material in the standard is considered proprietary information, however it does not contain any poisonous substance. Proclin 300 (1:3000) is used as preservative.

Information on reagents:
In most cases the stop solution provided is 1 N H2SO4. The formulation of wash solution is proprietary information. None of the components contain (sodium) azide, thimerosal, 2-mercaptoethanol (2-ME) or any other poisonous materials. For the sandwich method kits, the sample diluent, antibody diluent, enzyme diluent and standard all contain BSA.

Information on antibodies:
The antibodies provided in different kits vary in regards to clonality and host. Some antibodies are affinity purified, some are Protein A

Probenmenge: 100 μL

Testdauer: 1 - 4.5 h

Plattentyp: Pre-coated

Protokoll: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADMA has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADMA present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADMA is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADMA bound in the initial step. The color development is stopped and the intensity of the color is measured.

Testpräzision: Intra-assay precision (precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess precision.
Inter-assay precision (precision between assays): Three samples of known concentration were tested in twenty assays to assess precision.

• Intra-assay: CV% less than 8%
• Inter-assay: CV% less than 10%

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Vorsichtsmaßnahmen: The Stop Solution provided with this kit is an acid solution. Wear eye, hand, face and clothing protection when using this material.

Handhabung:

• The kit should not be used beyond the expiration date on the kit label.
• Do not mix or substitute reagents with those from other lots or sources.
• If samples generate values higher than the highest standard, dilute the samples with Sample Diluent and repeat the assay.
• Any variation in Sample Diluent, operator, pipetting technique, washing technique, incubation time/temperature and kit age can cause variation in binding.
• This assay is designed to eliminate interference by soluble receptors, binding proteins and other factors present in biological samples. Until all factors have been tested in the Immunoassay, the possibility of interference cannot be excluded.

Lagerung: 4 °C/-20 °C

Informationen zur Lagerung: For unopened kit: All the reagents should be kept according to the labels on vials.

Haltbarkeit: 6 months

Referenzen für ADMA ELISA Kit (ABIN367029)

Li, Zheng, Long, Zhang, Zhang, Tian, Zhou, Lv: "Effect of iloprost on biomarkers in patients with congenital heart disease-pulmonary arterial hypertension." in: Clinical and experimental pharmacology & physiology, Vol. 44, Issue 9, pp. 914-923, (2018) (PubMed).

de Giorgis, Marcovecchio, Giannini, Chiavaroli, Chiarelli, Mohn: "Blood pressure from childhood to adolescence in obese youths in relation to insulin resistance and asymmetric dimethylarginine." in: Journal of endocrinological investigation, (2015) (PubMed).

Marcovecchio, Gravina, Gallina, DAdamo, De Caterina, Chiarelli, Mohn, Renda: "Increased left atrial size in obese children and its association with insulin resistance: a pilot study." in: European journal of pediatrics, (2015) (PubMed).

De Marco, Marcovecchio, Caniglia, De Leonibus, Chiarelli, Mohn: "Circulating asymmetric dimethylarginine and lipid profile in pre-pubertal children with growth hormone deficiency: effect of 12-month growth hormone replacement therapy." in: Growth hormone & IGF research : official journal of the Growth Hormone Research Society and the International IGF Research Society, Vol. 24, Issue 5, pp. 216-20, (2014) (PubMed).

Chen, Zhou, Kuang, Tang, Li, Chen: "4-HNE increases intracellular ADMA levels in cultured HUVECs: evidence for miR-21-dependent mechanisms." in: PLoS ONE, Vol. 8, Issue 5, pp. e64148, (2013) (PubMed).

Sun, Zhou, Kuang, Li, Xiong, Tang, Xia, Bai, Yang, Li, Chen: "Correlations of DDAH1 transcript variants with human endothelial asymmetric dimethylarginine metabolizing activity." in: American journal of hypertension, Vol. 26, Issue 12, pp. 1437-44, (2013) (PubMed).

Ozturk, Karadag, Yegen, Gursu, Uzun, Aydin, Gurdal, Koldas, Kumbasar, Kazancioglu: "The relationship of plasma ADMA levels with cardiac functions and metabolic parameters in peritoneal dialysis patients." in: Clinical and experimental nephrology, Vol. 17, Issue 3, pp. 431-6, (2013) (PubMed).

Wei, He, Lv: "Effect of nisoldipine and olmesartan on endothelium-dependent vasodilation in essential hypertensive patients." in: CNS neuroscience & therapeutics, Vol. 18, Issue 5, pp. 400-5, (2012) (PubMed).

Details zu ADMA

Target: ADMA (Asymmetrical Dimethylarginine (ADMA))

Abstract: ADMA Produkte