C-JUN ELISA Kit

Produktnummer ABIN2866256

Human C-JUN ELISA-Kit Colorimetric-Assay zur Quantifizierung von Human C-JUN.

Kurzübersicht für C-JUN ELISA Kit (ABIN2866256)

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Reaktivität: Human

Nachweismethode: Colorimetric

Methodentyp: DNA-Binding ELISA

Applikation: ELISA

Proben: Cell Extracts, Tissue Samples

Produktdetails C-JUN ELISA Kit

Verwendungszweck: DNA-binding ELISA that facilitate the study of transcription factor activation in mammalien tissue and cell culture extracts.

Marke: TransAM®

Analytische Methode: Quantitative

Spezifität: The TransAM AP-1 Family Kit is tested for sensitivity and specifictiy in detecting AP-1 activation.

Produktmerkmale: Transcription factors are DNA-binding proteins that tightly regulate gene expression. They consist of two distinct domains - one that displays high affinity for a specific DNA sequence and one that confers transcriptional activity. Transcription factors are activated by phosphorylation of specific residues or by processing bound inhibitory proteins. Understanding and quantifying transcription factors is essential for the study of cell functions in relation to differentiation, brain activity, immune response, inflammation and various disease states. TransAM® Kits are sensitive, non-radioactive transcription factor ELISA kits that facilitate the study of transcription factor activation in mammalian tissue and cell extracts.
TransAM® Kits are DNA-binding ELISAs that facilitate the study of transcription factor activation in mammalian tissue and cell extracts. Each kit includes a 96-stripwell plate in which multiple copies of a specific double-stranded oligonucleotide have been immobilized. When nuclear or whole-cell extract is added, activated transcription factor of interest binds the oligonucleotide at its consensus binding site and is quantified using the included antibody, which is specific for the bound, active form of the transcription factor being studied.

Bestandteile: One or five 96-well plate(s) with plate sealer(s), primary antibody, HRP-conjugated secondary antibody, wild-type and mutated competitor oligonucleotides, positive control cell extract, DTT, Protease Inhibitor Cocktail, Lysis, Binding, 10X Washing and 10X Antibody Binding Buffers, and Developing and Stop Solutions.

Anwendungsinformationen

Applikationshinweise: Optimal working dilution should be determined by the investigator.

Kommentare:

Nuclear extracts are prepared from WI-38 VA13 cells that have been stimulated with PMA/Ionomycin. TransAM assays are performed using 5 μg/well of extract with the c-Jun, c-Fos, FosB, Fra-1, Fra-2, JunB and JunD antibodies found in the TransAM AP-1 Family Kit. The TransAM assays are performed in the absence or presence of a 20X excess of competitor oligonucleotides, which contain either a wild-type or mutated AP-1 consensus binding site (Figure 1). The wild-type oligonucleotide prevents AP-1 binding to the probe immobilized on the plate. Conversely, the mutated oligonucleotide has a limited effect on AP-1 binding The relative activation of the family members may vary depending on the cell type tested and the manner in which it was stimulated.

Testdauer: 5 h

Plattentyp: Pre-coated

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C/-20 °C/-80 °C

Informationen zur Lagerung: Except for the cell extract that must be kept at -80°C, the kit components can be stored at -20°C prior to first use. Then, we recommend storing the kit at 4°C except for the oligonucleotides, the c-Jun antibody, DTT, Poly [d(I-C)] and Protease Inhibitor Cocktail that should be kept at -20°C, and the cell extract at -80°C. This product is guaranteed for 6 months from date of receipt.

Haltbarkeit: 6 months

Details zu C-JUN

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Andere Bezeichnung: Ap-1

Pathways: MAPK Signalweg, RTK Signalweg, WNT Signalweg, Fc-epsilon Rezeptor Signalübertragung, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagie, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteine