Osteoprotegerin ELISA Kit

Produktnummer ABIN2748464

Produktdetails Osteoprotegerin ELISA Kit

Target: Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))

Reaktivität: Schwein

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.1-30 ng/mL

Untere Nachweisgrenze: 0.1 ng/mL

Applikation: ELISA

Verwendungszweck: Porcine Osteoprotegerin (TNFRSF11B) ELISA Kit for Cell Culture Supernatants, Plasma, and Serum samples.

Proben: Plasma, Cell Culture Supernatant, Serum

Analytische Methode: Quantitative

Spezifität: The antibody pair provided in this kit recognizes Porcine Osteoprotegerin (TNFRSF11B)

Sensitivität: 0.12 ng/mL

Produktmerkmale:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Bestandteile:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Benötigtes Material:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Anwendungsinformationen

Applikationshinweise: Recommended Dilution for serum and plasma samples2 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Testdurchführung:

Prepare all reagents, samples and standards as instructed in the manual.Add 100 μL of standard or sample to each well.Incubate 2.5 h at RT or O/N at 4 °C.Add 100 μL of prepared biotin antibody to each well.Incubate 1 h at RT.Add 100 μL of prepared Streptavidin solution to each well.Incubate 45 min at RT.Add 100 μL of TMB One-Step Substrate Reagent to each well.Incubate 30 min at RT.Add 50 μL of Stop Solution to each well.Read at 450 nm immediately.

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: -20 °C

Informationen zur Lagerung: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Haltbarkeit: 6 months

Details zu Osteoprotegerin

Target: Osteoprotegerin (TNFRSF11B) (Tumor Necrosis Factor Receptor Superfamily, Member 11b (TNFRSF11B))

Andere Bezeichnung: Osteoprotegerin (TNFRSF11B Produkte)

Synonyme: CD254 ELISA Kit, ODF ELISA Kit, OPGL ELISA Kit, OPTB2 ELISA Kit, RANKL ELISA Kit, TRANCE ELISA Kit, hRANKL2 ELISA Kit, sOdf ELISA Kit, OCIF ELISA Kit, OPG ELISA Kit, TR1 ELISA Kit, Opg ELISA Kit, ocif ELISA Kit, opg ELISA Kit, tr1 ELISA Kit, TNF superfamily member 11 ELISA Kit, TNF receptor superfamily member 11b ELISA Kit, tumor necrosis factor receptor superfamily, member 11b (osteoprotegerin) ELISA Kit, TNF receptor superfamily member 11B ELISA Kit, tumor necrosis factor receptor superfamily member 11b S homeolog ELISA Kit, tumor necrosis factor receptor superfamily, member 11b ELISA Kit, TNFSF11 ELISA Kit, TNFRSF11B ELISA Kit, Tnfrsf11b ELISA Kit, tnfrsf11b.S ELISA Kit

Substanzklasse: Chemical

Hintergrund: Osteoprotegerin (TNFRSF11B)

Gen-ID: 100738370

UniProt: F1S278