IgG2a ELISA Kit

Produktnummer ABIN2703581

Produktdetails IgG2a ELISA Kit

Target: IgG2a

Reaktivität: Maus

Nachweismethode: Colorimetric

Methodentyp: Sandwich ELISA

Detektionsbereich: 0.6-150 ng/mL

Untere Nachweisgrenze: 0.6 ng/mL

Applikation: ELISA

Verwendungszweck: Mouse IgG_2a ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse IgG_2a in serum, plasma, and cell culture supernatants.

Proben: Cell Culture Supernatant, Plasma, Serum

Analytische Methode: Quantitative

Spezifität: This ELISA antibody pair detects mouse heavy chain of IgG2a. Other species not determined.

Sensitivität: 0.6 ng/ml

Bestandteile: 96 wells (12 strips x 8 wells) Coated With Antibody
Wash Buffer Concentrate (20x)
Positive Control
Assay Diluent
Detection Antibody
Secondary Antibody or HRP Streptavidin
TMB One-Step Substrate Reagent
Stop Solution
Cell Lysis Buffer

Benötigtes Material: Distilled or deionized water
100 mL and 1 liter graduated cylinders
Tubes to prepare sample dilutions
Protease and Phosphatase inhibitors
Precision pipettes to deliver 2 μL to 1 mL volumes
Adjustable 1-25 mL pipettes for reagent preparation
Benchtop rocker or shaker
Microplate reader capable of measuring absorbance at 450 nm

Anwendungsinformationen

Applikationshinweise: Optimal working dilution: 10,000 fold

Probenmenge: 100 μL

Plattentyp: Pre-coated

Protokoll: This assay employs an antibody specific for mouse IgG_2a coated on a 96-well plate. Standards and samples are pipetted into the wells and IgG_2a present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse IgG_2a antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IgG_2a bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Aufbereitung der Proben:

For example, add 2 μL of serum or plasma into a tube with 198.0 μL 1X Assay Diluent to prepare a 100-fold diluted sample. Mix thoroughly and then pipette 3 μL of prepared 100-fold diluted sample into a tube with 297 μL 1X Assay Dil

Beschränkungen: Nur für Forschungszwecke einsetzbar

Handhabung

Lagerung: 4 °C

Haltbarkeit: 6 months

Details zu IgG2a

Target: IgG2a

Abstract: IgG2a Produkte

Synonyme: gamma-2a immunoglobulin heavy chain ELISA Kit, IgG-2a ELISA Kit

Substanzklasse: Antibody