Recommended Dilution for serum and plasma samples2,000 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual.
Add 100 μL of standard or sample to each well.
Incubate 2.5 h at RT or O/N at 4 °C.
Add 100 μL of prepared biotin antibody to each well.
Incubate 1 h at RT.
Add 100 μL of prepared Streptavidin solution to each well.
Incubate 45 min at RT.
Add 100 μL of TMB One-Step Substrate Reagent to each well.
Incubate 30 min at RT.
Add 50 μL of Stop Solution to each well.
Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
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Małkiewicz, Myśliwska, Skrzypkowska, Słomiński, Siebert, Gutknecht: "Vascular endothelial growth factor polymorphism (-460 T/C) is related to hypertension-associated chronic kidney disease." in: Clinical and experimental hypertension (New York, N.Y. : 1993), Vol. 38, Issue 5, pp. 469-75, (2016) (PubMed).
Małkiewicz, Skrzypkowska, Słomiński, Siebert, Gutknecht, Myśliwska: "The GG genotype of the -152 G/A vascular endothelial growth factor (VEGF) polymorphism predisposes to hypertension-related chronic kidney disease." in: Molecular biology reports, Vol. 43, Issue 9, pp. 967-75, (2016) (PubMed).
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Zhai, Chen, Gao, Ma, Chen, Yu, Li, Liu, Xiang, Xie, Yang: "Inhibition of the NF-κB pathway by R65 ribozyme gene via adeno-associated virus serotype 9 ameliorated oxidized LDL induced human umbilical vein endothelial cell injury." in: International journal of clinical and experimental pathology, Vol. 8, Issue 9, pp. 9912-21, (2015) (PubMed).