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HAVCR1 ELISA Kit
HAVCR1
Reaktivität: Ratte
Colorimetric
Sandwich ELISA
10-3.000 pg/mL
Cell Culture Supernatant, Plasma, Serum
Produktnummer ABIN2703497
Produktdetails HAVCR1 ELISA Kit
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Target
Alle HAVCR1 ELISA Kits anzeigen
HAVCR1
(Hepatitis A Virus Cellular Receptor 1 (HAVCR1))
Reaktivität
Alle Reaktivitäten für HAVCR1 ELISA Kits
Ratte
Nachweismethode
Colorimetric
Methodentyp
Sandwich ELISA
Detektionsbereich
10-3.000 pg/mL
Untere Nachweisgrenze
10 pg/mL
Applikation
ELISA
Verwendungszweck
Rat TIM-1 (KIM-1) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Proben
Cell Culture Supernatant, Plasma, Serum
Analytische Methode
Quantitative
Spezifität
This ELISA antibody pair detects rat TIM-1, no cross-reactivity with mouse TIM 1, TIM-3, TIM-4, human TIM 1 or TIM-4 is observed.
Sensitivität
10 pg/mL
Produktmerkmale
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Bestandteile
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Benötigtes Material
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Anwendungsinformationen
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Applikationshinweise
Recommended Dilution for serum and plasma samples2 fold
Probenmenge
100 μL
Plattentyp
Pre-coated
Protokoll
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Beschränkungen
Nur für Forschungszwecke einsetzbar
Handhabung
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Lagerung
-20 °C
Informationen zur Lagerung
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Haltbarkeit
6 months
Details zu HAVCR1
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Target
Alle HAVCR1 ELISA Kits anzeigen
HAVCR1
(Hepatitis A Virus Cellular Receptor 1 (HAVCR1))
Andere Bezeichnung
TIM-1 (HAVCR1 Produkte )
Synonyme
HAVCR ELISA Kit, HAVCR-1 ELISA Kit, KIM-1 ELISA Kit, KIM1 ELISA Kit, TIM ELISA Kit, TIM-1 ELISA Kit, TIM1 ELISA Kit, TIMD-1 ELISA Kit, TIMD1 ELISA Kit, Kim1 ELISA Kit, HAVCR1 ELISA Kit, LOC100226241 ELISA Kit, AI503787 ELISA Kit, Tim1 ELISA Kit, Timd1 ELISA Kit, hepatitis A virus cellular receptor 1 ELISA Kit, hepatitis A virus cellular receptor 1 homolog ELISA Kit, HAVCR1 ELISA Kit, Havcr1 ELISA Kit, LOC100226241 ELISA Kit
Substanzklasse
Virus
Hintergrund
Gene Names: Havcr1 Kim1 Protein names: Hepatitis A virus cellular receptor 1 homolog (HAVcr-1) (Kidney injury molecule 1) (KIM-1) (T cell immunoglobulin and mucin domain-containing protein 1) (TIMD-1)
Gen-ID
286934
UniProt
O54947
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